Production of hydrogen peroxide and hydroxyl radical in potato tuber during the necrotrophic phase of hemibiotrophic pathogen Phytophthora infestans infection
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23142933
DOI
10.1016/j.jphotobiol.2012.10.001
PII: S1011-1344(12)00219-9
Knihovny.cz E-resources
- MeSH
- Hydroxyl Radical metabolism MeSH
- Food Quality MeSH
- Molecular Imaging MeSH
- Plant Diseases parasitology MeSH
- Hydrogen Peroxide metabolism MeSH
- Lipid Peroxidation MeSH
- Phytophthora infestans growth & development physiology MeSH
- Solanum tuberosum metabolism parasitology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hydroxyl Radical MeSH
- Hydrogen Peroxide MeSH
In this study, evidence is provided on the formation of hydrogen peroxide (H(2)O(2)) and hydroxyl radical (HO) in the potato tuber during the necrotrophic phase of the hemibiotrophic pathogen Phytophthora infestans infection. Using 3,3-diaminobenzidine tetrahydrochloride (DAB) imaging technique, the formation of H(2)O(2) was demonstrated in P. infestans-infected potato tuber. For the first time, HO formation was demonstrated in P. infestans-infected potato tuber using electron paramagnetic resonance (EPR) spectroscopy. An enhancement in spontaneous ultra-weak photon emission indicated the extent of lipid peroxidation in the P. infestans-infected potato tuber. The data presented in this study reveal that the formation of H(2)O(2) and HO in the P. infestans-infected potato tuber is associated with lipid peroxidation. It is proposed here that the ultra-weak photon emission can be used as a non-invasive indicator of the oxidative processes in the quality control at food industry.
References provided by Crossref.org
Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds
