Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 Å.

Faculty of Fisheries and Protection of Waters University of South Bohemia in Ceske Budejovice CENAKVA and Institute of Complex Systems Zamek 136 373 33 Nove Hrady Czech Republic

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Banáš, P., Otyepka, M., Jeřábek, P., Petřek, M. & Damborský, J. (2006). PubMed

Bidmanova, S., Chaloupkova, R., Damborsky, J. & Prokop, Z. (2010). PubMed

Bogdanović, X., Hesseler, M., Palm, G. J., Bornscheuer, U. T. & Hinrichs, W. (2010). PubMed PMC

Campbell, D. W., Müller, C. & Reardon, K. F. (2006). PubMed

Chovancová, E., Kosinski, J., Bujnicki, J. M. & Damborský, J. (2007). PubMed

Degtjarik, O., Chaloupkova, R., Rezacova, P., Kuty, M., Damborsky, J. & Kuta Smatanova, I. (2013). PubMed PMC

Diederichs, K. & Karplus, P. A. (1997). PubMed

Drienovska, I., Chovancova, E., Koudelakova, T., Damborsky, J. & Chaloupkova, R. (2012). PubMed PMC

Ducruix, A. & Giegé, R. (1999).

Gehret, J. J., Gu, L., Geders, T., Brown, W. C., Gerwick, L., Gerwick, W. H., Sherman, D. H. & Smith, J. L. (2012). PubMed PMC

Hesseler, M., Bogdanovic, X., Hidalgo, A., Berenguer, J., Palm, G. J., Hinrichs, W. & Bornscheuer, U. T. (2011). PubMed

Janssen, D. B. (2004).

Janssen, D. B., Dinkla, I. J., Poelarends, G. J. & Terpstra, P. (2005). PubMed

Kabsch, W. (2010). PubMed PMC

Koudelakova, T., Bidmanova, S., Dvorak, P., Pavelka, A., Chaloupkova, R., Prokop, Z. & Damborsky, J. (2013). PubMed

Koudelakova, T., Chovancova, E., Brezovsky, J., Monincova, M., Fortova, A., Jarkovsky, J. & Damborsky, J. (2011). PubMed

Krug, M., Weiss, M. S., Heinemann, U. & Mueller, U. (2012).

Los, G. V. & Wood, K. (2007). PubMed

Matthews, B. W. (1968). PubMed

Minor, W., Cymborowski, M., Otwinowski, Z. & Chruszcz, M. (2006). PubMed

Mueller, U., Darowski, N., Fuchs, M. R., Förster, R., Hellmig, M., Paithankar, K. S., Pühringer, S., Steffien, M., Zocher, G. & Weiss, M. S. (2012). PubMed PMC

Newman, J., Peat, T. S., Richard, R., Kan, L., Swanson, P. E., Affholter, J. A., Holmes, I. H., Schindler, J. F., Unkefer, C. J. & Terwilliger, T. C. (1999). PubMed

Ohana, R. F., Encell, L. P., Zhao, K., Simpson, D., Slater, M. R., Urh, M. & Wood, K. V. (2009). PubMed

Ollis, D. L., Cheah, E., Cygler, M., Dijkstra, B., Frolow, F., Franken, S. M., Harel, M., Remington, S. J., Silman, I. & Schrag, J. D. (1992). PubMed

Poelarends, G. J., Zandstra, M., Bosma, T., Kulakov, L. A., Larkin, M. J., Marchesi, J. R., Weightman, A. J. & Janssen, D. B. (2000). PubMed PMC

Prokop, Z., Sato, Y., Brezovsky, J., Mozga, T., Chaloupkova, R., Koudelakova, T., Jerabek, P., Stepankova, V., Natsume, R., van Leeuwen, J., Janssen, D., Florian, J., Nagata, Y., Senda, T. & Damborsky, J. (2010). PubMed

Prudnikova, T., Mozga, T., Rezacova, P., Chaloupkova, R., Sato, Y., Nagata, Y., Brynda, J., Kuty, M., Damborsky, J. & Kuta Smatanova, I. (2009). PubMed PMC

Ridder, I. S., Rozeboom, H. J. & Dijkstra, B. W. (1999). PubMed

Stsiapanava, A., Chaloupkova, R., Fortova, A., Brynda, J., Weiss, M. S., Damborsky, J. & Kuta Smatanova, I. (2011). PubMed PMC

Stsiapanava, A., Dohnalek, J., Gavira, J. A., Kuty, M., Koudelakova, T., Damborsky, J. & Kuta Smatanova, I. (2010). PubMed

Stucki, G. & Thueer, M. (1995). PubMed

Vagin, A. & Teplyakov, A. (2010). PubMed

Weiss, M. S. & Hilgenfeld, R. (1997).

Weiss, M. S., Metzner, H. J. & Hilgenfeld, R. (1998). PubMed

Westerbeek, A., Szymański, W., Feringa, B. L. & Janssen, D. B. (2011).

Winn, M. D.

Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17

Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5