Plasma treated polyethylene grafted with adhesive molecules for enhanced adhesion and growth of fibroblasts
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23827550
DOI
10.1016/j.msec.2012.12.003
PII: S0928-4931(12)00560-7
Knihovny.cz E-resources
- Keywords
- Biomolecules grafting, Mouse embryonic fibroblasts, Plasma treatment, Polyethylene, Surface characterization,
- MeSH
- Adhesiveness drug effects MeSH
- Cell Adhesion drug effects MeSH
- NIH 3T3 Cells MeSH
- Time Factors MeSH
- Nitrogen analysis MeSH
- Fibroblasts cytology MeSH
- Microscopy, Fluorescence MeSH
- Photoelectron Spectroscopy MeSH
- Cells, Cultured MeSH
- Oxygen analysis MeSH
- Microscopy, Atomic Force MeSH
- Mice MeSH
- Plasma Gases pharmacology MeSH
- Cell Count MeSH
- Polyethylene pharmacology MeSH
- Cell Proliferation drug effects MeSH
- Wettability drug effects MeSH
- Carbon analysis MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nitrogen MeSH
- Oxygen MeSH
- Plasma Gases MeSH
- Polyethylene MeSH
- Carbon MeSH
The cell-material interface plays a crucial role in the interaction of cells with synthetic materials for biomedical use. The application of plasma for tailoring polymer surfaces is of abiding interest and holds a great promise in biomedicine. In this paper, we describe polyethylene (PE) surface tuning by Ar plasma irradiating and subsequent grafting of the chemically active PE surface with adhesive proteins or motives to support cell attachment. These simple modifications resulted in changed polymer surface hydrophilicity, roughness and morphology, which we thoroughly characterized. The effect of our modifications on adhesion and growth was tested in vitro using mouse embryonic fibroblasts (NIH 3T3 cell line). We demonstrate that the plasma treatment of PE had a positive effect on the adhesion, spreading, homogeneity of distribution and moderately on proliferation activity of NIH 3T3 cells. This effect was even more pronounced on PE coated with biomolecules.
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