Surface characterization
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Plasma discharge was used to modify the ETFE surface properties. The changes induced by various plasma power and exposure time were determined by goniometry, gravimetric analysis and atomic force microscopy. It was found that a significant decrease of contact angle after plasma exposure is connected with the surface oxygen increase. Plasma treatment also induced a significant ablation of ETFE and fragmentation of its lamellar structure. The disruption of the lamellar structure was more pronounced for higher plasma power. It was found that by varying plasma power and exposure time it is possible to induce selective surface changes which can subsequently be used for various tissue engineering applications.
Polymeric biomaterials with antibacterial effects are requisite materials in the fight against hospital-acquired infections. An effective way for constructing a second generation of antibacterials is to exploit the synergic effect of (i) patterning of polymeric materials by a laser, and (ii) deposition of noble metals in their nanostructured forms. With this approach, we prepared highly-ordered periodic structures (ripples) on polyethylene naphthalate (PEN). Subsequent deposition of Ag under the glancing angle of 70° resulted in the formation of self-organized, fully separated Ag nanowire (Ag NW) arrays homogenously distributed on PEN surface. Surface properties of these samples were characterized by AFM and XPS. Vacuum evaporation of Ag at the glancing angle geometry of 70° caused that Ag NWs were formed predominantly from one side of the ripples, near to the top of the ridges. The release of Ag(+) ions into physiological solution was studied by ICP-MS. The results of antibacterial tests predetermine these novel structures as promising materials able to fight against a broad spectrum of microorganisms, however, their observed cytotoxicity warns about their applications in the contact with living tissues.
- MeSH
- antibakteriální látky chemie farmakologie MeSH
- Escherichia coli účinky léků MeSH
- fotoelektronová spektroskopie MeSH
- lasery * MeSH
- mikroskopie atomárních sil MeSH
- nanodráty chemie toxicita MeSH
- polyethylen chemie MeSH
- povrchové vlastnosti MeSH
- Staphylococcus epidermidis účinky léků MeSH
- stříbro chemie MeSH
- Publikační typ
- časopisecké články MeSH
1st ed. 18, 298 s.
- MeSH
- biofyzika MeSH
- buněčná membrána MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Buněčná biologie. Cytologie
- NLK Obory
- cytologie, klinická cytologie
- fyzika, biofyzika
Current topics in pathology, ISSN 0070-2188 83, 1991
XII, 522 s. : il., tab.
We report a new approach to characterization of thin (bio)molecular films based on spectroscopy of Bragg-scattered surface plasmons (BSSPs) generated by diffraction-coupling of counterpropagating surface plasmons on a metal-coated diffraction grating. The BSSPs exhibit fields with different penetration depths into the medium adjacent to the metal and therefore exhibit unequal sensitivities to the presence of (bio)molecular films on the surface of the metal. Therefore, spectroscopy of BSSPs enables in situ observation of the formation of biomolecular films and determination of both their refractive index and thickness. We demonstrate this capacity of spectroscopy of BSSPs in a model experiment in which growth of protein layers on a gold surface is studied.
- MeSH
- biomedicínský výzkum MeSH
- elektrická stimulace MeSH
- elektromyografie MeSH
- hemiplegie patofyziologie MeSH
- lidé MeSH
- nervus ischiadicus fyziologie MeSH
- nervus medianus fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH