A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cytochrome P-450 CYP3A genetics metabolism MeSH
- HeLa Cells MeSH
- Humans MeSH
- Molecular Probes genetics metabolism MeSH
- Pregnane X Receptor MeSH
- Response Elements * MeSH
- Electrophoretic Mobility Shift Assay methods MeSH
- Receptors, Steroid genetics metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytochrome P-450 CYP3A MeSH
- Molecular Probes MeSH
- Pregnane X Receptor MeSH
- Receptors, Steroid MeSH
The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA–protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ³²P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences.
References provided by Crossref.org
Acetylation of lysine 109 modulates pregnane X receptor DNA binding and transcriptional activity
