Polysome profile analysis--yeast
Language English Country United States Media print
Document type Journal Article
PubMed
24034321
DOI
10.1016/b978-0-12-420037-1.00009-9
PII: B978-0-12-420037-1.00009-9
Knihovny.cz E-resources
- Keywords
- Grow yeast cultures, Polysome profile analysis, Sucrose density gradient centrifugation, Western and Northern blotting, Yeast whole cell extracts (WCEs),
- MeSH
- Centrifugation, Density Gradient methods MeSH
- Blotting, Northern methods MeSH
- Polyribosomes chemistry genetics MeSH
- Saccharomyces cerevisiae chemistry cytology genetics MeSH
- Sucrose chemistry MeSH
- Blotting, Western methods MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Sucrose MeSH
More than one 80S monosome can translate an mRNA molecule at a time producing polysomes. The most widely used method to separate 40S and 60S ribosomal subunits from 80S monosomes and polysomes is a high-velocity centrifugation of whole cell extracts in linear sucrose gradients. This polysome profile analysis technique has been routinely used to monitor translational fitness of cells under a variety of physiological conditions, to investigate functions of initiation factors involved in translation, to reveal defects in ribosome biogenesis, to determine roles of 5' UTR structures on mRNA translatability, and more recently for examination of miRNA-mediated translational repression (see an application of this protocol on Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).
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