3D printed chip for electrochemical detection of influenza virus labeled with CdS quantum dots
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24296063
DOI
10.1016/j.bios.2013.10.031
PII: S0956-5663(13)00733-1
Knihovny.cz E-resources
- Keywords
- 3D Printing, Influenza hemagglutinin, Microfluidic chip, Paramagnetic particles, Quantum dots, Voltammetry,
- MeSH
- Betainfluenzavirus isolation & purification MeSH
- Biosensing Techniques instrumentation MeSH
- Equipment Design MeSH
- Hemagglutinins, Viral isolation & purification MeSH
- Orthomyxoviridae Infections virology MeSH
- Quantum Dots chemistry MeSH
- Humans MeSH
- Microfluidic Analytical Techniques instrumentation MeSH
- Orthomyxoviridae isolation & purification MeSH
- Cadmium Compounds chemistry MeSH
- Selenium Compounds chemistry MeSH
- Influenza Vaccines analysis MeSH
- Influenza A Virus, H1N1 Subtype isolation & purification MeSH
- Influenza A Virus, H3N2 Subtype isolation & purification MeSH
- Influenza A virus isolation & purification MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- cadmium selenide MeSH Browser
- Hemagglutinins, Viral MeSH
- Cadmium Compounds MeSH
- Selenium Compounds MeSH
- Influenza Vaccines MeSH
In this study, we report a new three-dimensional (3D), bead-based microfluidic chip developed for rapid, sensitive and specific detection of influenza hemagglutinin. The principle of microfluidic chip is based on implementation of two-step procedure that includes isolation based on paramagnetic beads and electrochemical detection. As a platform for isolation process, streptavidin-modified MPs, which were conjugated via biotinylated glycan (through streptavidin-biotin affinity) followed by linkage of hemagglutinin to glycan, were used. Vaccine hemagglutinin (HA vaxi) was labeled with CdS quantum dots (QDs) at first. Detection of the isolation product by voltammetry was the end point of the procedure. The suggested and developed method can be used also for detection of other specific substances that are important for control, diagnosis or therapy of infectious diseases.
References provided by Crossref.org
Concept and Evolution in 3D Printing for Excellence in Healthcare
Recent advances in lab-on-a-chip technologies for viral diagnosis
Advanced nanotechnologies in avian influenza: Current status and future trends - A review
Ultrasensitive detection of influenza viruses with a glycan-based impedimetric biosensor
