Diagnosis of Epstein-Barr virus infection in clinical serum samples by an SPR biosensor assay
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24389391
DOI
10.1016/j.bios.2013.12.011
PII: S0956-5663(13)00880-4
Knihovny.cz E-resources
- Keywords
- Antifouling, Epstein–Barr virus infection, Polymer brushes, Real time diagnostics, Surface plasmon resonance biosensor,
- MeSH
- Equipment Failure Analysis MeSH
- Biosensing Techniques instrumentation MeSH
- Equipment Design MeSH
- Immunoassay instrumentation MeSH
- Epstein-Barr Virus Infections blood diagnosis immunology MeSH
- Humans MeSH
- Surface Plasmon Resonance instrumentation MeSH
- Antibodies, Viral immunology MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antibodies, Viral MeSH
Label-free affinity biosensors offer a promising platform for the development of a new generation of medical diagnostic technologies. Nevertheless, when such sensors are used in complex biological media, adsorption of non-targeted medium components prevents the specific detection of the analyte. In this work, we introduce for the first time a biosensor assay based on surface plasmon resonance (SPR) capable of diagnosing different stages of Epstein-Barr virus (EBV) infections in clinical serum samples. This was achieved by simultaneous detection of the antibodies against three different antigens present in the virus. To prevent the interference of the fouling from serum during the measurement, the SPR chips were coated by an antifouling layer of a polymer brush of poly[oligo(ethylene glycol) methacrylate] grown by surface-initiated atom transfer radical polymerization. The bioreceptors were then attached via hybridization of complementary oligonucleotides. This allowed the sensor surface to be regenerated after measurement by disrupting the complementary pairs above the oligonucleotides' melting temperature and attaching new bioreceptors. In this way, the same sensing surface could be used repeatedly. The procedure used in this work will serve as a prototype strategy for the development of label-free affinity biosensors for diagnostics in blood serum or plasma samples. This is the first example of detection of marker of a disease in clinical serum samples by an optical affinity biosensor.
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