Highly undesirable microbial contaminants of processed cheese are endospore-forming bacteria of the genera Bacillus and Clostridium. Survival of Bacillus subtilis, B. cereus, Clostridium butyricum and C. sporogenes was examined in model processed cheese samples supplemented with monoacylglycerols. In processed cheese samples, monoacylglycerols of undecanoic, undecenoic, lauric and adamantane-1-carboxylic acid at concentration of 0.15% w/w prevented the growth and multiplication of both Bacillus species throughout the storage period. The two species of Clostridium were less affected by monoacylglycerols in processed cheese samples and only partial inhibition was observed. The effect of milk fat content on microbial survival in processed cheese was also evaluated. The growth of Bacillus sp. was affected by the fat level of processed cheese while population levels of Clostridium sp. did not differ in processed cheese samples with 30, 40 and 50% fat in dry matter.

Department of Environmental Protection Engineering Faculty of Technology Tomas Bata University in Zlín nám T G Masaryka 275 76272 Zlín Czech Republic

Department of Fat Tenside and Cosmetics Technology Faculty of Technology Tomas Bata University in Zlín nám T G Masaryka 275 76272 Zlín Czech Republic

Department of Food Technology Faculty of Technology Tomas Bata University in Zlín nám T G Masaryka 275 76272 Zlín Czech Republic

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