Complete sequences of IncHI1 plasmids carrying blaCTX-M-1 and qnrS1 in equine Escherichia coli provide new insights into plasmid evolution
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24862095
DOI
10.1093/jac/dku172
PII: dku172
Knihovny.cz E-zdroje
- Klíčová slova
- CTX-M-1, IncHI1, IncX1, fructooligosaccharides, horses,
- MeSH
- beta-laktamasy genetika MeSH
- Escherichia coli genetika izolace a purifikace MeSH
- infekce vyvolané Escherichia coli mikrobiologie veterinární MeSH
- koně MeSH
- metabolické sítě a dráhy genetika MeSH
- molekulární evoluce * MeSH
- molekulární sekvence - údaje MeSH
- plazmidy * MeSH
- pořadí genů MeSH
- proteiny z Escherichia coli genetika MeSH
- sekvenční analýza DNA * MeSH
- syntenie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- beta-lactamase TEM-3 MeSH Prohlížeč
- beta-laktamasy MeSH
- proteiny z Escherichia coli MeSH
- Qnr protein, E coli MeSH Prohlížeč
OBJECTIVES: To determine the structure of two multidrug-resistant IncHI1 plasmids carrying blaCTX-M-1 in Escherichia coli isolates disseminated in an equine clinic in the Czech Republic. METHODS: A complete nucleotide sequencing of 239 kb IncHI1 (pEQ1) and 287 kb IncHI1/X1 (pEQ2) plasmids was performed using the 454-Genome Sequencer FLX system. The sequences were compared using bioinformatic tools with other sequenced IncHI1 plasmids. RESULTS: A comparative analysis of pEQ1 and pEQ2 identified high nucleotide identity with the IncHI1 type 2 plasmids. A novel 24 kb module containing an operon involved in short-chain fructooligosaccharide uptake and metabolism was found in the pEQ backbones. The role of the pEQ plasmids in the metabolism of short-chain fructooligosaccharides was demonstrated by studying the growth of E. coli cells in the presence of these sugars. The module containing the blaCTX-M-1 gene was formed by a truncated macrolide resistance cluster and flanked by IS26 as previously observed in IncI1 and IncN plasmids. The IncHI1 plasmid changed size and gained the quinolone resistance gene qnrS1 as a result of IS26-mediated fusion with an IncX1 plasmid. CONCLUSIONS: Our data highlight the structure and evolution of IncHI1 from equine E. coli. A plasmid-mediated sugar metabolic element could play a key role in strain fitness, contributing to the successful dissemination and maintenance of these plasmids in the intestinal microflora of horses.
Citace poskytuje Crossref.org
GENBANK
KF362121, KF362122
