Complete sequences of IncHI1 plasmids carrying blaCTX-M-1 and qnrS1 in equine Escherichia coli provide new insights into plasmid evolution
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24862095
DOI
10.1093/jac/dku172
PII: dku172
Knihovny.cz E-resources
- Keywords
- CTX-M-1, IncHI1, IncX1, fructooligosaccharides, horses,
- MeSH
- beta-Lactamases genetics MeSH
- Escherichia coli genetics isolation & purification MeSH
- Escherichia coli Infections microbiology veterinary MeSH
- Horses MeSH
- Metabolic Networks and Pathways genetics MeSH
- Evolution, Molecular * MeSH
- Molecular Sequence Data MeSH
- Plasmids * MeSH
- Gene Order MeSH
- Escherichia coli Proteins genetics MeSH
- Sequence Analysis, DNA * MeSH
- Synteny MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- beta-lactamase TEM-3 MeSH Browser
- beta-Lactamases MeSH
- Escherichia coli Proteins MeSH
- Qnr protein, E coli MeSH Browser
OBJECTIVES: To determine the structure of two multidrug-resistant IncHI1 plasmids carrying blaCTX-M-1 in Escherichia coli isolates disseminated in an equine clinic in the Czech Republic. METHODS: A complete nucleotide sequencing of 239 kb IncHI1 (pEQ1) and 287 kb IncHI1/X1 (pEQ2) plasmids was performed using the 454-Genome Sequencer FLX system. The sequences were compared using bioinformatic tools with other sequenced IncHI1 plasmids. RESULTS: A comparative analysis of pEQ1 and pEQ2 identified high nucleotide identity with the IncHI1 type 2 plasmids. A novel 24 kb module containing an operon involved in short-chain fructooligosaccharide uptake and metabolism was found in the pEQ backbones. The role of the pEQ plasmids in the metabolism of short-chain fructooligosaccharides was demonstrated by studying the growth of E. coli cells in the presence of these sugars. The module containing the blaCTX-M-1 gene was formed by a truncated macrolide resistance cluster and flanked by IS26 as previously observed in IncI1 and IncN plasmids. The IncHI1 plasmid changed size and gained the quinolone resistance gene qnrS1 as a result of IS26-mediated fusion with an IncX1 plasmid. CONCLUSIONS: Our data highlight the structure and evolution of IncHI1 from equine E. coli. A plasmid-mediated sugar metabolic element could play a key role in strain fitness, contributing to the successful dissemination and maintenance of these plasmids in the intestinal microflora of horses.
References provided by Crossref.org
GENBANK
KF362121, KF362122
