Absence of maternal cell contamination in mesenchymal stromal cell cultures derived from equine umbilical cord tissue
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24889156
DOI
10.1016/j.placenta.2014.04.005
PII: S0143-4004(14)00157-X
Knihovny.cz E-resources
- Keywords
- Maternal cell contamination, Mesenchymal stromal cells, Microsatellite markers, Umbilical cord tissue,
- MeSH
- Horses * MeSH
- Cells, Cultured * MeSH
- Mesenchymal Stem Cells * MeSH
- Microsatellite Repeats MeSH
- Umbilical Cord cytology MeSH
- Pregnancy MeSH
- Animals MeSH
- Check Tag
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This study aimed to determine whether maternal cell contamination exists in cells derived from equine umbilical cord tissue, a perspective material for cell-based therapies in veterinary medicine. Potential maternal cell contamination was analyzed at DNA level via a set of 16 microsatellite markers in cells originating from the cord tissue of 22 foals. In these cells no maternal cell contamination was detected at a sensitivity level of 0.01%. Our results suggest that equine umbilical cord tissue-derived cells are entirely of fetal origin.
Department of Animal Husbandry Czech University of Life Sciences Prague Czech Republic
Department of Biology of Reproduction Institute of Animal Science Prague Czech Republic
Department of Molecular Genetics Institute of Animal Science Prague Czech Republic
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