Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24906923
DOI
10.1016/j.domaniend.2014.01.003
PII: S0739-7240(14)00004-6
Knihovny.cz E-resources
- Keywords
- Granulosa, Indomethacin, Oocyte, Progesterone, RU486,
- MeSH
- Hormone Antagonists pharmacology MeSH
- C-Reactive Protein genetics metabolism MeSH
- Extracellular Matrix metabolism MeSH
- Follicle Stimulating Hormone MeSH
- Indomethacin pharmacology MeSH
- Cyclooxygenase Inhibitors pharmacology MeSH
- In Vitro Oocyte Maturation Techniques veterinary MeSH
- Cumulus Cells drug effects physiology MeSH
- Hyaluronic Acid MeSH
- Luteinizing Hormone MeSH
- Mifepristone pharmacology MeSH
- Cell Adhesion Molecules genetics metabolism MeSH
- Oocytes drug effects physiology MeSH
- Swine * MeSH
- Progesterone metabolism MeSH
- Gene Expression Regulation drug effects MeSH
- Serum Amyloid P-Component genetics metabolism MeSH
- Carrier Proteins genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hormone Antagonists MeSH
- C-Reactive Protein MeSH
- Follicle Stimulating Hormone MeSH
- Indomethacin MeSH
- Cyclooxygenase Inhibitors MeSH
- Hyaluronic Acid MeSH
- Luteinizing Hormone MeSH
- Mifepristone MeSH
- Cell Adhesion Molecules MeSH
- Progesterone MeSH
- PTX3 protein MeSH Browser
- Serum Amyloid P-Component MeSH
- Carrier Proteins MeSH
This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan (HA) and heavy chains of inter-α trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor α-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were based on freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100 ng/mL) in the presence/absence of RU486 or indomethacin. Western blot analysis, (3)H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 μM) caused a decrease in the number of oocytes that reached germinal vesicle breakdown and metaphase II stage compared with indomethacin (100 μM) or FSH/LH treatment alone after 44 h. All treated OCCs synthesized an almost equal amount of HA. Heavy chains (of inter-α trypsin inhibitor)-HA covalent complexes were formed during in vitro FSH/LH-stimulated expansion in RU486- or indomethacin-treated OCCs. Follicle-stimulating hormone/LH-induced progesterone production by OCCs was increased in the presence of RU486 after 44 h. In contrast, a decrease of FSH/LH-stimulated progesterone production by GCs was detected in the presence of either RU486 or indomethacin after 72 h. We suggest that the PR-dependent pathway may be involved in the regulation of oocyte maturation. Both PR and COX-2 regulate FSH/LH-stimulated progesterone production by OCCs and GCs.
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