Effects of RU486 and indomethacin on meiotic maturation, formation of extracellular matrix, and progesterone production by porcine oocyte-cumulus complexes
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24906923
DOI
10.1016/j.domaniend.2014.01.003
PII: S0739-7240(14)00004-6
Knihovny.cz E-zdroje
- Klíčová slova
- Granulosa, Indomethacin, Oocyte, Progesterone, RU486,
- MeSH
- antagonisté hormonů farmakologie MeSH
- C-reaktivní protein genetika metabolismus MeSH
- extracelulární matrix metabolismus MeSH
- folikuly stimulující hormon MeSH
- indomethacin farmakologie MeSH
- inhibitory cyklooxygenasy farmakologie MeSH
- IVM techniky veterinární MeSH
- kumulární buňky účinky léků fyziologie MeSH
- kyselina hyaluronová MeSH
- luteinizační hormon MeSH
- mifepriston farmakologie MeSH
- molekuly buněčné adheze genetika metabolismus MeSH
- oocyty účinky léků fyziologie MeSH
- prasata * MeSH
- progesteron metabolismus MeSH
- regulace genové exprese účinky léků MeSH
- sérový amyloidový protein genetika metabolismus MeSH
- transportní proteiny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antagonisté hormonů MeSH
- C-reaktivní protein MeSH
- folikuly stimulující hormon MeSH
- indomethacin MeSH
- inhibitory cyklooxygenasy MeSH
- kyselina hyaluronová MeSH
- luteinizační hormon MeSH
- mifepriston MeSH
- molekuly buněčné adheze MeSH
- progesteron MeSH
- PTX3 protein MeSH Prohlížeč
- sérový amyloidový protein MeSH
- transportní proteiny MeSH
This study was designed to determine whether inhibition of either cyclooxygenase-2 (COX-2) by indomethacin or progesterone receptor (PR) by PR antagonist, RU486, affects oocyte maturation, progesterone production, and covalent binding between hyaluronan (HA) and heavy chains of inter-α trypsin inhibitor, as well as expression of cumulus expansion-associated proteins (HA-binding protein, tumor necrosis factor α-induced protein 6, pentraxin 3) in oocyte-cumulus complexes (OCCs). The experiments were based on freshly isolated porcine OCC cultures in which the consequences of PR and COX-2 inhibition on the final processes of oocyte maturation were determined. Granulosa cells (GCs) and OCCs were cultured in medium supplemented with FSH/LH (both 100 ng/mL) in the presence/absence of RU486 or indomethacin. Western blot analysis, (3)H-glucosamine hydrochloride assay, immunofluorescence, and radioimmunoassay were performed. Only treatment with RU486 (25 μM) caused a decrease in the number of oocytes that reached germinal vesicle breakdown and metaphase II stage compared with indomethacin (100 μM) or FSH/LH treatment alone after 44 h. All treated OCCs synthesized an almost equal amount of HA. Heavy chains (of inter-α trypsin inhibitor)-HA covalent complexes were formed during in vitro FSH/LH-stimulated expansion in RU486- or indomethacin-treated OCCs. Follicle-stimulating hormone/LH-induced progesterone production by OCCs was increased in the presence of RU486 after 44 h. In contrast, a decrease of FSH/LH-stimulated progesterone production by GCs was detected in the presence of either RU486 or indomethacin after 72 h. We suggest that the PR-dependent pathway may be involved in the regulation of oocyte maturation. Both PR and COX-2 regulate FSH/LH-stimulated progesterone production by OCCs and GCs.
Citace poskytuje Crossref.org
