A sentinel protein assay for simultaneously quantifying cellular processes
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25194849
DOI
10.1038/nmeth.3101
PII: nmeth.3101
Knihovny.cz E-zdroje
- MeSH
- fosfoproteiny chemie MeSH
- hmotnostní spektrometrie metody MeSH
- lidé MeSH
- mapování interakce mezi proteiny MeSH
- peptidy chemie MeSH
- proteomika metody MeSH
- reprodukovatelnost výsledků MeSH
- Saccharomyces cerevisiae - proteiny chemie MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- systémová biologie metody MeSH
- transkriptom MeSH
- výpočetní biologie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfoproteiny MeSH
- peptidy MeSH
- Saccharomyces cerevisiae - proteiny MeSH
We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.
Institute of Biochemistry Department of Biology ETH Zurich Zurich Switzerland
Institute of Molecular Systems Biology Department of Biology ETH Zurich Zurich Switzerland
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