Selected ⁶⁸Ga-siderophores versus ⁶⁸Ga-colloid and ⁶⁸Ga-citrate: biodistribution and small animal imaging in mice
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
I 1346
Austrian Science Fund FWF - Austria
L 676
Austrian Science Fund FWF - Austria
PubMed
25363728
PubMed Central
PMC4843960
DOI
10.5507/bp.2014.052
Knihovny.cz E-resources
- MeSH
- Aspergillosis diagnostic imaging metabolism MeSH
- Citrates pharmacokinetics MeSH
- Peptides, Cyclic pharmacokinetics MeSH
- Diagnostic Imaging * MeSH
- Gallium pharmacokinetics MeSH
- Heterocyclic Compounds, 1-Ring pharmacokinetics MeSH
- Colloids MeSH
- Mice, Inbred DBA MeSH
- Mice MeSH
- Positron-Emission Tomography methods MeSH
- Radiopharmaceuticals pharmacokinetics MeSH
- Gallium Radioisotopes MeSH
- Siderophores MeSH
- Somatostatin analogs & derivatives pharmacokinetics MeSH
- Tissue Distribution MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Citrates MeSH
- Peptides, Cyclic MeSH
- Gallium MeSH
- gallium 68-DOTA-lanreotide MeSH Browser
- gallium citrate MeSH Browser
- Heterocyclic Compounds, 1-Ring MeSH
- Colloids MeSH
- Radiopharmaceuticals MeSH
- Gallium Radioisotopes MeSH
- Siderophores MeSH
- Somatostatin MeSH
BACKGROUND: (68)Ga-triacetylfusarinine C (TAFC) and (68)Ga-ferrioxamine E (FOXE) show great potential to be used as highly sensitive and selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution and small animal imaging of (68)Ga-TAFC and (68)Ga-FOXE versus (68)Ga-colloid and (68)Ga-citrate as unspecific control in mice. METHODS: The radiochemical purity of tested (68)Ga labelled tracers was determined by RP-HPLC or ITLC-SG. Ex vivo biodistribution was studied in normal DBA/2 mice 30 min and 90 min p.i. Static and dynamic imaging were performed using µPET/CT. RESULTS: (68)Ga-TAFC and (68)Ga-FOXE showed rapid renal excretion and low blood values even 90 min p.i. (68)Ga-TAFC showed almost no retention in other organs while (68)Ga-FOXE displayed some uptake in gastrointestinal tract. (68)Ga-colloid and (68)Ga-citrate revealed significantly different ex vivo biodistribution. (68)Ga-colloid showed pronounced radioactivity retention in the liver, while (68)Ga-citrate displayed high blood values and significant retention of radioactivity in highly perfused organs. CONCLUSIONS: From the results, both (68)Ga-TAFC and (68)Ga-FOXE have excellent and significantly different in vivo behaviour compared to (68)Ga-colloid and (68)Ga-citrate. (68)Ga-TAFC in particular confirmed its great potential use as a specific tracer for Aspergillus infection imaging.
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Neilands JB. Siderophores: structure and function of microbial iron transport compounds. J Biol Chem. 1995;270:26723–6. PubMed
Hider RC, Kong X. Chemistry and biology of siderophores. Nat Prod Rep. 2010;27:637–57. PubMed
Weiss G. Iron and immunity: a double-edged sword. Eur J Clin Invest. 2002;32:70–8. PubMed
Schrettl M, Beckmann N, Varga J, Heinekamp T, Jacobsen ID, Jöchl C, Moussa TA, Wang S, Gsaller F, Blatzer M, Werner ER, et al. HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus. PLoSPathog. 2010;6(9):e1001124. doi: 10.1371/journal.ppat.1001124. PubMed DOI PMC
Baddley JW, Andes DR, Marr KA, Kontoyiannis DP, Alexander BD, Kauffman CA, et al. Factors associated with mortality in transplant patients with invasive aspergillosis. Clin Infect Dis. 2010;50:1559–67. PubMed PMC
Pagano L, Akova M, Dimopoulos G, Herbrecht R, Drgona L, Blijlevens N. Risk assessment and prognostic factors for mouldrelated diseases in immunocompromised patients. J Antimicrob Chemother. 2011;66:i5–14. PubMed
Reichenberger F, Habicht JM, Gratwohl A, Tamm M. Diagnosis and treatment of invasive pulmonary aspergillosis in neutropenic patients. Eur Respir J. 2002;19:743–55. PubMed
Petrik M, Haas H, Schrettl M, Helbok A, Blatzer M, Decristoforo C. In vitro and in vivo evaluation of selected 68Ga-siderophores for infection imaging. Nucl Med Biol. 2012;39:361–9. PubMed PMC
Fani M, André JP, Maecke HR. 68Ga-PET: a powerful generator based alternative to cyclotron-based PET radiopharmaceuticals. Contrast Media Mol Imaging. 2008;3:53–63. PubMed
Decristoforo C. Gallium-68 – a new opportunity for PET available from a long shelf-life generator – automation and applications. Curr Radiopharm. 2012;5:212–20. PubMed
Velikyan I. Prospective of 68Ga-radiopharmaceutical development. Theranostics. 2014;4:47–80. PubMed PMC
Petrik M, Haas H, Dobrozemsky G, Lass-Flörl C, Helbok A, Blatzer M, Dietrich H, Decristoforo C. 68Ga-Siderophores for PET imaging of invasive pulmonary aspergillosis: proof of principle. J Nucl Med. 2010;51:639–45. PubMed PMC
Petrik M, Franssen GM, Haas H, Laverman P, Hörtnagl C, Schrettl M, Helbok A, Lass-Flörl C, Decristoforo C. Preclinical evaluation of two 68Ga-siderophores as potential radiopharmaceuticals for Aspergillus fumigatus infection imaging. Eur J Nucl Med Mol Imaging. 2012;39:1175–83. PubMed PMC
Rizzello A, Di Pierro D, Lodi F, Trespidi S, Cicoria G, Pancaldi D, Nanni C, Marengo M, Marzola MC, Al-Nahhas A, Rubello D, et al. Synthesis and quality control of 68Ga citrate for routine clinical PET. Nucl Med Comm. 2009;30:542–5. PubMed
Ocak M, Antretter M, Knopp R, Kunkel F, Petrik M, Bergisadi N, Decristoforo C. Full automation of 68Ga labelling of DOTA-peptides including cation exchange prepurification. Appl Radiat Isot. 2010;68:297–302. PubMed
Sánchez F, Orero A, Soriano A, Correcher C, Conde P, González A, Hernández L, Moliner L, Rodríguez-Alvarez MJ, Vidal LF, Benlloch JM, et al. ALBIRA: a small animal PET/SPECT/CT imaging system. Med Phys. 2013;40:051906. PubMed
Sanchez F, Moliner L, Correcher C, Gonzalez A, Orero A, Carles M, Soriano A, Rodriguez-Alvarez MJ, Medina LA, Mora F, Benlloch JM. Small animal PET scanner based on monolithic LYSO crystals: performance evaluation. Med Phys. 2012;39:643–53. PubMed
Rösch F. Past, present and future of 68Ge/68Ga generators. Appl Radiat Isot. 2013;76:24–30. PubMed
Petrik M, Haas H, Laverman P, Schrettl M, Franssen GM, Blatzer M, Decristoforo C. 68Ga-Triacetylfusarinine C and 68Ga-Ferrioxamine E for Aspergillus infection imaging: uptake specificity in various microorganisms. Mol Imaging Biol. 2014;16:102–8. PubMed PMC
Hughes DK. Nuclear medicine and infection detection: the relative effectiveness of imaging with 111In-oxine-, 99mTc-HMPAO-, and 99mTc-stannous fluoride colloid-labeled leukocytes and with 67Ga-citrate. J Nucl Med Technol. 2003;31:196–201. PubMed
Nanni C, Errani C, Boriani L, Fantini L, Ambrosini V, Boschi S, Rubello D, Pettinato C, Mercuri M, Gasbarrini A, Fanti S. 68Ga-citrate PET/CT for evaluating patients with infections of the bone: preliminary results. J Nucl Med. 2010;51:1932–6. PubMed
Kumar V, Boddeti DK, Evans SG, Angelides S. 68Ga-Citrate-PET for diagnostic imaging of infection in rats and for intra-abdominal infection in a patient. Curr Radiopharm. 2012;5:71–5. PubMed
Silvola JM, Laitinen I, Sipilä HJ, Laine VJ, Leppänen P, Ylä-Herttuala S, Knuuti J, Roivainen A. Uptake of 68gallium in atherosclerotic plaques in LDLR-/-ApoB100/100 mice. EJNMMI Res. 2011;1(1):14. doi: 10.1186/2191-219X-1-14. PubMed DOI PMC
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