Expression of clock genes period and timeless in the central nervous system of the Mediterranean flour moth, Ephestia kuehniella
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25637625
DOI
10.1177/0748730414568430
PII: 0748730414568430
Knihovny.cz E-zdroje
- Klíčová slova
- Lepidoptera, PER-like immunoreactivity, activity rhythms, circadian clock, eclosion rhythm, timeless,
- MeSH
- biologické hodiny genetika MeSH
- cirkadiánní hodiny genetika MeSH
- cirkadiánní proteiny Period genetika metabolismus MeSH
- cirkadiánní rytmus genetika fyziologie MeSH
- fenotyp MeSH
- fotoperioda MeSH
- hmyzí proteiny genetika MeSH
- hybridizace in situ MeSH
- imunohistochemie MeSH
- jaderné proteiny metabolismus MeSH
- klonování organismů MeSH
- komplementární DNA MeSH
- messenger RNA genetika metabolismus MeSH
- mozek metabolismus MeSH
- můry genetika růst a vývoj fyziologie MeSH
- regulace genové exprese MeSH
- sekvenční analýza DNA MeSH
- světlo MeSH
- zrakový lalok nesavčí fyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cirkadiánní proteiny Period MeSH
- hmyzí proteiny MeSH
- jaderné proteiny MeSH
- komplementární DNA MeSH
- messenger RNA MeSH
Homologous circadian genes are found in all insect clocks, but their contribution to species-specific circadian timing systems differs. The aim of this study was to extend research within Lepidoptera to gain a better understanding of the molecular mechanism underlying circadian clock plasticity and evolution. The Mediterranean flour moth, Ephestia kuehniella (Pyralidae), represents a phylogenetically ancestral lepidopteran species. We have identified circadian rhythms in egg hatching, adult emergence, and adult locomotor activity. Cloning full-length complementary DNAs and further characterization confirmed one copy of period and timeless genes in both sexes. Both per and tim transcripts oscillate in their abundance in E. kuehniella heads under light-dark conditions. PER-like immunoreactivity (PER-lir) was observed in nuclei and cytoplasm of most neurons in the central brain, the ventral part of subesophageal complex, the neurohemal organs, the optic lobes, and eyes. PER-lir in photoreceptor nuclei oscillated during the day with maximal intensity in the light phase of the photoperiodic regime and lack of a signal in the middle of the dark phase. Expression patterns of per and tim messenger RNAs (mRNAs) were revealed in the identical location as the PER-lir was detected. In the photoreceptors, a daily rhythm in the intensity of expression of both per mRNA and tim mRNA was found. These findings suggest E. kuehniella as a potential lepidopteran model for circadian studies.
Citace poskytuje Crossref.org
