Expression of clock genes period and timeless in the central nervous system of the Mediterranean flour moth, Ephestia kuehniella
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25637625
DOI
10.1177/0748730414568430
PII: 0748730414568430
Knihovny.cz E-resources
- Keywords
- Lepidoptera, PER-like immunoreactivity, activity rhythms, circadian clock, eclosion rhythm, timeless,
- MeSH
- Biological Clocks genetics MeSH
- Circadian Clocks genetics MeSH
- Period Circadian Proteins genetics metabolism MeSH
- Circadian Rhythm genetics physiology MeSH
- Phenotype MeSH
- Photoperiod MeSH
- Insect Proteins genetics MeSH
- In Situ Hybridization MeSH
- Immunohistochemistry MeSH
- Nuclear Proteins metabolism MeSH
- Cloning, Organism MeSH
- DNA, Complementary MeSH
- RNA, Messenger genetics metabolism MeSH
- Brain metabolism MeSH
- Moths genetics growth & development physiology MeSH
- Gene Expression Regulation MeSH
- Sequence Analysis, DNA MeSH
- Light MeSH
- Optic Lobe, Nonmammalian physiology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Period Circadian Proteins MeSH
- Insect Proteins MeSH
- Nuclear Proteins MeSH
- DNA, Complementary MeSH
- RNA, Messenger MeSH
Homologous circadian genes are found in all insect clocks, but their contribution to species-specific circadian timing systems differs. The aim of this study was to extend research within Lepidoptera to gain a better understanding of the molecular mechanism underlying circadian clock plasticity and evolution. The Mediterranean flour moth, Ephestia kuehniella (Pyralidae), represents a phylogenetically ancestral lepidopteran species. We have identified circadian rhythms in egg hatching, adult emergence, and adult locomotor activity. Cloning full-length complementary DNAs and further characterization confirmed one copy of period and timeless genes in both sexes. Both per and tim transcripts oscillate in their abundance in E. kuehniella heads under light-dark conditions. PER-like immunoreactivity (PER-lir) was observed in nuclei and cytoplasm of most neurons in the central brain, the ventral part of subesophageal complex, the neurohemal organs, the optic lobes, and eyes. PER-lir in photoreceptor nuclei oscillated during the day with maximal intensity in the light phase of the photoperiodic regime and lack of a signal in the middle of the dark phase. Expression patterns of per and tim messenger RNAs (mRNAs) were revealed in the identical location as the PER-lir was detected. In the photoreceptors, a daily rhythm in the intensity of expression of both per mRNA and tim mRNA was found. These findings suggest E. kuehniella as a potential lepidopteran model for circadian studies.
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