NOD/SCID IL2Rγ-null mouse xenograft model of human p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma using permanent cell lines
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Chronic lymphocytic leukemia, NSG mouse, mantle cell lymphoma, xenograft model,
- MeSH
- ATM protein genetika MeSH
- biologické markery MeSH
- chronická lymfatická leukemie genetika metabolismus patologie MeSH
- genový knockout MeSH
- heterografty MeSH
- lidé MeSH
- lymfom z plášťových buněk genetika patologie MeSH
- modely nemocí na zvířatech MeSH
- mutace * MeSH
- myši inbrední NOD MeSH
- myši knockoutované MeSH
- myši SCID MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- nádorový supresorový protein p53 genetika MeSH
- receptory interleukinů - společná gama-podjednotka genetika MeSH
- transplantace heterologní MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ATM protein MeSH
- biologické markery MeSH
- nádorový supresorový protein p53 MeSH
- receptory interleukinů - společná gama-podjednotka MeSH
Xenograft models represent a promising tool to study the pathogenesis of hematological malignancies. To establish a reliable and appropriate in vivo model of aggressive human B-cell leukemia and lymphoma we xenotransplanted four p53-mutated cell lines and one ATM-mutated cell line into immunodeficient NOD/SCID IL2Rγ-null mice. The cell lines MEC-1, SU-DHL-4, JEKO-1, REC-1, and GRANTA-519 were transplanted intraperitoneally or subcutaneously and the engraftment was investigated using immunohistochemistry and flow cytometry. We found significant differences in engraftment efficiency. MEC-1, JEKO-1 and GRANTA-519 cell lines engrafted most efficiently, while SU-DHL-4 cells did not engraft at all. MEC-1 and GRANTA-519 massively infiltrated organs and the whole intraperitoneal cavity showing very aggressive growth. In addition, GRANTA-519 cells massively migrated to the bone marrow regardless of the transplantation route. The MEC-1 and GRANTA-519 cells can be especially recommended for in vivo study of p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma, respectively.
b CEITEC Central European Institute of Technology Masaryk University Brno Czech Republic
c Department of Pathology University Hospital Brno Czech Republic
Department of Pathology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic
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