NOD/SCID IL2Rγ-null mouse xenograft model of human p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma using permanent cell lines
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Chronic lymphocytic leukemia, NSG mouse, mantle cell lymphoma, xenograft model,
- MeSH
- Ataxia Telangiectasia Mutated Proteins genetics MeSH
- Biomarkers MeSH
- Leukemia, Lymphocytic, Chronic, B-Cell genetics metabolism pathology MeSH
- Gene Knockout Techniques MeSH
- Heterografts MeSH
- Humans MeSH
- Lymphoma, Mantle-Cell genetics pathology MeSH
- Disease Models, Animal MeSH
- Mutation * MeSH
- Mice, Inbred NOD MeSH
- Mice, Knockout MeSH
- Mice, SCID MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Tumor Suppressor Protein p53 genetics MeSH
- Interleukin Receptor Common gamma Subunit genetics MeSH
- Transplantation, Heterologous MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ataxia Telangiectasia Mutated Proteins MeSH
- Biomarkers MeSH
- Tumor Suppressor Protein p53 MeSH
- Interleukin Receptor Common gamma Subunit MeSH
Xenograft models represent a promising tool to study the pathogenesis of hematological malignancies. To establish a reliable and appropriate in vivo model of aggressive human B-cell leukemia and lymphoma we xenotransplanted four p53-mutated cell lines and one ATM-mutated cell line into immunodeficient NOD/SCID IL2Rγ-null mice. The cell lines MEC-1, SU-DHL-4, JEKO-1, REC-1, and GRANTA-519 were transplanted intraperitoneally or subcutaneously and the engraftment was investigated using immunohistochemistry and flow cytometry. We found significant differences in engraftment efficiency. MEC-1, JEKO-1 and GRANTA-519 cell lines engrafted most efficiently, while SU-DHL-4 cells did not engraft at all. MEC-1 and GRANTA-519 massively infiltrated organs and the whole intraperitoneal cavity showing very aggressive growth. In addition, GRANTA-519 cells massively migrated to the bone marrow regardless of the transplantation route. The MEC-1 and GRANTA-519 cells can be especially recommended for in vivo study of p53-mutated chronic lymphocytic leukemia and ATM-mutated mantle cell lymphoma, respectively.
b CEITEC Central European Institute of Technology Masaryk University Brno Czech Republic
c Department of Pathology University Hospital Brno Czech Republic
Department of Pathology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic
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