Quantitative analysis of volatile metabolites released in vitro by bacteria of the genus Stenotrophomonas for identification of breath biomarkers of respiratory infection in cystic fibrosis
Language English Country Great Britain, England Media electronic
Document type Journal Article
- MeSH
- 1-Propanol analysis metabolism MeSH
- Ammonia analysis metabolism MeSH
- Biomarkers analysis MeSH
- Cystic Fibrosis complications MeSH
- Breath Tests methods MeSH
- Disulfides analysis metabolism MeSH
- Genotype MeSH
- Gram-Negative Bacterial Infections complications diagnosis metabolism MeSH
- Mass Spectrometry methods MeSH
- Respiratory Tract Infections complications diagnosis metabolism MeSH
- Humans MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Stenotrophomonas chemistry metabolism MeSH
- In Vitro Techniques MeSH
- Volatile Organic Compounds analysis metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 1-Propanol MeSH
- Ammonia MeSH
- Biomarkers MeSH
- dimethyl disulfide MeSH Browser
- Disulfides MeSH
- Volatile Organic Compounds MeSH
The aim of the present study was to characterize the volatile metabolites produced by genotypically diverse strains of the Stenotrophomonas genus in order to evaluate their potential as biomarkers of lung infection by non-invasive breath analysis. Volatile organic compounds (VOCs) emitted from 15 clinical and five environmental strains belonging to different genogroups of Stenotrophomonas maltophilia (n = 18) and Stenotrophomonas rhizophila (n = 2) cultured in Mueller-Hinton Broth (MHB) liquid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several VOCs were detected in high concentration, including ammonia, propanol, dimethyl disulphide propanol and dimethyl disulphide. The GC-MS measurements showed that all 15 clinical strains produced similar headspace VOCs compositions, and SIFT-MS quantification showed that the rates of production of the VOCs by the genotypically distinct strains were very similar. All in vitro cultures of both the Stenotrophomonas species were characterised by efficient production of two isomers of methyl butanol, which can be described by known biochemical pathways and which is absent in other pathogens, including Pseudomonas aeruginosa. These in-vitro data indicate that methyl butanol isomers may be exhaled breath biomarkers of S. maltophilia lung infection in patients with cystic fibrosis.
References provided by Crossref.org
Recent developments and applications of selected ion flow tube mass spectrometry (SIFT-MS)
