We investigated concerted evolution of rRNA genes in multiple populations of Tragopogon mirus and T. miscellus, two allotetraploids that formed recurrently within the last 80 years following the introduction of three diploids (T. dubius, T. pratensis, and T. porrifolius) from Europe to North America. Using the earliest herbarium specimens of the allotetraploids (1949 and 1953) to represent the genomic condition near the time of polyploidization, we found that the parental rDNA repeats were inherited in roughly equal numbers. In contrast, in most present-day populations of both tetraploids, the rDNA of T. dubius origin is reduced and may occupy as little as 5% of total rDNA in some individuals. However, in two populations of T. mirus the repeats of T. dubius origin outnumber the repeats of the second diploid parent (T. porrifolius), indicating bidirectional concerted evolution within a single species. In plants of T. miscellus having a low rDNA contribution from T. dubius, the rDNA of T. dubius was nonetheless expressed. We have apparently caught homogenization of rDNA repeats (concerted evolution) in the act, although it has not proceeded to completion in any allopolyploid population yet examined.

Institute of Biophysics Academy of Sciences of the Czech Republic CS 61265 Brno Czech Republic

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Abbott, R. J., and A. J. Lowe, 2004. Origins, establishment and evolution of two new polyploid species of

Ainouche, M. L., A. Baumel and A. Salmon, 2004.

Álvarez, I., and J. F. Wendel, 2003. Ribosomal ITS sequences and plant phylogenetic inference. Mol. Phylogenet. Evol. 29 PubMed

Bennett, R. I., and A. G. Smith, 1991. Use of a genomic clone for ribosomal RNA from PubMed

Brehm, B. G., and M. Ownbey, 1965. Variation in chromatographic patterns in the

Brown, R. K., and C. G. Schaak, 1972. Two new species of

Buckler, E. S., IV, A. Ippolito and T. P. Holtsford, 1997. The evolution of ribosomal DNA: divergent paralogues and phylogenetic implications. Genetics 145 PubMed PMC

Chase, M. W., S. Knapp, A. V. Cox, J. J. Clarkson, Y. Butsko PubMed PMC

Chen, Z. J., and C. S. Pikaard, 1997. Epigenetic silencing of RNA polymerase I transcription: a role for DNA methylation and histone modification in nucleolar dominance. Genes Dev. 11 PubMed PMC

Coleman, A. W., 2002. Comparison of Eudorina/Pleodorina ITS sequences of isolates from nature with those from experimental hybrids. Am. J. Bot. 89 PubMed

Cook, L. M., P. S. Soltis, S. J. Brunsfeld and D. E. Soltis, 1998. Multiple independent formations of

Cronn, R., M. Cedroni, T. Haselkorn, C. Grover and J. F. Wendel, 2002. PCR-mediated recombination in amplification products derived from polyploid cotton. Theor. Appl. Genet. 104 PubMed

Dover, G. A., 1982. Molecular drive: a cohesive mode of species evolution. Nature 299 PubMed

Flavell, R. B., and M. O'Dell, 1976. Ribosomal RNA genes in homeologous chromosomes of groups 5 and 6 in hexaploid wheat. Heredity 37

Franzke, A., and K. Mummenhoff, 1999. Recent hybrid speciation in

Gerlach, W. L., and J. R. Bedbrook, 1979. Cloning and characterization of ribosomal RNA genes in wheat. Nucleic Acids Res. 7 PubMed PMC

Gill, B. S., 1991 Nucleocytoplasmic interaction (NCI) hypothesis of genome evolution and speciation in polyploid plants, pp. 48–53 in

Hemleben, V., and U. Zentgraf, 1994 Structural organisation and regulation of transcription by RNA polymerase I of plant nuclear ribosomal genes, pp. 3–24 in PubMed

Hillis, D. M., C. Moritz, C. A. Porter and R. J. Baker, 1991. Evidence for biased gene conversion in concerted evolution of ribosomal DNA. Science 251 PubMed

Hughes, K. W., and R. H. Petersen, 2001. Apparent recombination or gene conversion in the ribosomal ITS region of a PubMed

Joly, S., J. T. Rauscher, S. L. Sherman-Broyles, A. H. D. Brown and J. J. Doyle, 2004. Evolutionary dynamics and preferential expression of homeologous 18S–5.8S–26S nuclear ribosomal genes in natural and artificial PubMed

Kim, K. J., and R. K. Jansen, 1994. Comparisons of phylogenetic hypotheses among different data sets in dwarf dandelions (

Kiss, T., A. Szkukalek and F. Solymosy, 1989. Nucleotide sequence of a 17S (18S) rRNA gene from tomato. Nucleic Acids Res. 17 PubMed PMC

Koch, M. A., C. Dobes and T. Mitchell-Olds, 2003. Multiple hybrid formation in natural populations: concerted evolution of the internal transcribed spacer of nuclear ribosomal DNA (ITS) in North American Arabis divricarpa (Brassicaceae). Mol. Biol. Evol. 20 PubMed

Kotseruba, V., D. Gernand, A. Meister and A. Houben, 2003. Uniparental loss of ribosomal DNA in the allotetraploid grass PubMed

Kovarik, A., R. Matyasek, K. Y. Lim, K. Skalická, B. Koukalova

Leitch, A. R., K. Y. Lim, D. R. Webb and G. I. Mcfadden, 2001 In situ hybridisation in plant cell biology, pp. 267–293 in

Leitch, I. J., and M. D. Bennett, 1997. Polyploidy in angiosperms. Trends Plant Sci. 2

Lim, K. Y., A. Kovarik, R. Matyasek, M. Bezdek, C. P. Lichtenstein PubMed

Matzke, M., O. M. Scheid and A. J. Matzke, 1999. Rapid structural and epigenetic changes in polyploid and aneuploid genomes. BioEssays 21 PubMed

Mavrodiev, E. V., M. E. Tancig, M. A. Gitzendanner, J. Rocca, P. S. Soltis

Novak, S. J., D. E. Soltis and P. S. Soltis, 1991. Ownbey's Tragopogons: forty years later. Am. J. Bot. 78

O'Kane, S. L., B. A. Schaal and I. A. Al-Shehbaz, 1996. The origins of

Osborn, T. C, J. C. Pires, J. A. Birchler, D. L. Auger, Z. J. Chen PubMed

Ownbey, M., 1950. Natural hybridization and amphiploidy in the genus

Ownbey, M., and G. D. McCollum, 1953. Cytoplasmic inheritance and reciprocal amphiploidy in

Ownbey, M., and G. D. McCollum, 1954. The chromosomes of

Pires, J. C., K. Y. Lim, A. Kovarik, R. Matyasek, A. Boyd PubMed

Rambaut, A., 2003

Rauscher, J. T., J. J. Doyle and A. H. Brown, 2004. Multiple origins and nrDNA internal transcribed spacer homeologue evolution in the PubMed PMC

Rieseberg, L. H., 2001. Polyploid evolution: keeping the peace at genomic reunions. Curr. Biol. 11 PubMed

Roose, M. L., and L. D. Gottlieb, 1976. Genetic and biochemical consequences of polyploidy in PubMed

Skalická, K., K. Y. Lim, R. Matyášek, B. Koukalová, A. Leitch PubMed

Soltis, D. E., and P. S. Soltis, 1989. Allopolyploid speciation in

Soltis, D. E., P. S. Soltis, J. C. Pires, A. Kovarik and J. Tate, 2004. Recent and recurrent polyploidy in

Soltis, P. E., G. M. Plunkett, S. J. Novak and D. E. Soltis, 1995. Genetic variation in Tragopogon species: additional origins of the allotetraploids T. mirus and T. miscellus (Compositae). Am. J. Bot. 82

Soltis, P. S., and D. E. Soltis, 1991. Multiple origins of the allotetraploid Tragopogon mirus (Compositae): rDNA evidence. Syst. Bot. 16

Soltis, P. S., and D. E. Soltis, 2000. The role of genetic and genomic attributes in the success of polyploids. Proc. Natl. Acad. Sci. USA 97 PubMed PMC

Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins, 1997. The Clustal PubMed PMC

Urbanska, K. M., H. Hurka, E. Landolt, B. Neuffer and K. Mummenhoff, 1997. Hybridization and evolution in

Vanhouten, W. H. J., N. Scarlett and K. Bachmann, 1993. Nuclear-DNA markers of the Australian tetraploid microseris-scapigera and its North-American diploid relatives. Theor. Appl. Genet. 87 PubMed

Volkov, R. A., N. V. Borisjuk, I. I. Panchuk, D. Schweizer and V. Hemleben, 1999. Elimination and rearrangement of parental rDNA in the allotetraploid PubMed

Wen, J., and E. A. Zimmer, 1996. Phylogeny and biogeography of PubMed

Wendel, J. F., A. Schnabel and T. Seelanan, 1995. a Bidirectional interlocus concerted evolution following allopolyploid speciation in cotton ( PubMed PMC

Wendel, J. F., A. Schnabel and T. Seelanan, 1995. b An unusual ribosomal DNA sequence from PubMed

Zhang, D., and T. Sang, 1999. Physical mapping of ribosomal RNA genes in peonies ( PubMed

Zimmer, E. A., S. L. Martin, S. M. Beverley, Y. W. Kan and A. C. Wilson, 1980. Rapid duplication and loss of genes coding for the α chains of hemoglobin. Proc. Natl. Acad. Sci. USA 77 PubMed PMC

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