The aim of the present study was to characterize the volatile metabolites produced by genotypically diverse strains of the Stenotrophomonas genus in order to evaluate their potential as biomarkers of lung infection by non-invasive breath analysis. Volatile organic compounds (VOCs) emitted from 15 clinical and five environmental strains belonging to different genogroups of Stenotrophomonas maltophilia (n = 18) and Stenotrophomonas rhizophila (n = 2) cultured in Mueller-Hinton Broth (MHB) liquid media were analysed by gas chromatography mass spectrometry (GC-MS) and selected ion flow tube mass spectrometry (SIFT-MS). Several VOCs were detected in high concentration, including ammonia, propanol, dimethyl disulphide propanol and dimethyl disulphide. The GC-MS measurements showed that all 15 clinical strains produced similar headspace VOCs compositions, and SIFT-MS quantification showed that the rates of production of the VOCs by the genotypically distinct strains were very similar. All in vitro cultures of both the Stenotrophomonas species were characterised by efficient production of two isomers of methyl butanol, which can be described by known biochemical pathways and which is absent in other pathogens, including Pseudomonas aeruginosa. These in-vitro data indicate that methyl butanol isomers may be exhaled breath biomarkers of S. maltophilia lung infection in patients with cystic fibrosis.
- MeSH
- 1-propanol analýza metabolismus MeSH
- amoniak analýza metabolismus MeSH
- biologické markery analýza MeSH
- cystická fibróza komplikace MeSH
- dechové testy metody MeSH
- disulfidy analýza metabolismus MeSH
- genotyp MeSH
- gramnegativní bakteriální infekce komplikace diagnóza metabolismus MeSH
- hmotnostní spektrometrie metody MeSH
- infekce dýchací soustavy komplikace diagnóza metabolismus MeSH
- lidé MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- Stenotrophomonas chemie metabolismus MeSH
- techniky in vitro MeSH
- těkavé organické sloučeniny analýza metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l(-1), respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l(-1) h(-1), respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.
- MeSH
- 1-propanol metabolismus MeSH
- cytoplazma metabolismus MeSH
- ethery cyklické metabolismus MeSH
- furosemid farmakologie MeSH
- hexokinasa analýza MeSH
- krysa rodu rattus MeSH
- kyselina ethakrynová farmakologie MeSH
- L-laktátdehydrogenasa analýza MeSH
- laktáty analýza MeSH
- ledviny enzymologie chemie metabolismus MeSH
- polythiazid farmakologie MeSH
- pyruváty analýza MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
