Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25930965
DOI
10.1007/s00394-015-0910-5
PII: 10.1007/s00394-015-0910-5
Knihovny.cz E-resources
- Keywords
- Angiogenesis, Apoptosis, Cell proliferation, Fruit polyphenols, MCF-7, Mammary carcinogenesis, Rat,
- MeSH
- Ki-67 Antigen genetics metabolism MeSH
- Apoptosis drug effects MeSH
- Mammary Neoplasms, Experimental drug therapy MeSH
- Flavonoids analysis pharmacology MeSH
- DNA Fragmentation drug effects MeSH
- Antineoplastic Agents, Phytogenic analysis pharmacology MeSH
- Caspase 3 genetics metabolism MeSH
- Rats MeSH
- Humans MeSH
- Methylnitrosourea toxicity MeSH
- MCF-7 Cells MeSH
- Disease Models, Animal MeSH
- Fruit chemistry MeSH
- Polyphenols analysis pharmacology MeSH
- Cell Proliferation drug effects MeSH
- bcl-2-Associated X Protein genetics metabolism MeSH
- Vascular Endothelial Growth Factor Receptor-2 genetics metabolism MeSH
- Stilbenes analysis pharmacology MeSH
- Tyrosine analogs & derivatives metabolism MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3-nitrotyrosine MeSH Browser
- Ki-67 Antigen MeSH
- Bax protein, rat MeSH Browser
- Casp3 protein, rat MeSH Browser
- Flavin7 MeSH Browser
- Flavonoids MeSH
- Antineoplastic Agents, Phytogenic MeSH
- Caspase 3 MeSH
- Kdr protein, rat MeSH Browser
- Methylnitrosourea MeSH
- Polyphenols MeSH
- bcl-2-Associated X Protein MeSH
- Vascular Endothelial Growth Factor Receptor-2 MeSH
- Stilbenes MeSH
- Tyrosine MeSH
PURPOSE: Fruit and vegetable intake is inversely correlated with cancer; thus, it is proposed that an extract of phytochemicals as present in whole fruits, vegetables, or grains may have anti-carcinogenic properties. Thus, the anti-tumour effects of fruit peel polyphenols (Flavin7) in the chemoprevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis in female rats were evaluated. METHODS: Lyophilized substance of Flavin7 (F7) was administered at two concentrations of 0.3 and 3 % through diet. The experiment was terminated 14 weeks after carcinogen administration, and mammary tumours were removed and prepared for histopathological and immunohistochemical analysis. In addition, using an in vitro cytotoxicity assay, apoptosis and proliferation after F7 treatment in human breast adenocarcinoma (MCF-7) cells were performed. RESULTS: High-dose F7 suppressed tumour frequency by 58 % (P < 0.001), tumour incidence by 24 % (P < 0.05), and lengthened latency by 8 days (P > 0.05) in comparison with the control rats, whereas lower dose of F7 was less effective. Histopathological analysis of tumours showed significant decrease in the ratio of high-/low-grade carcinomas after high-dose F7 treatment. Immunohistochemical analysis of rat carcinoma cells in vivo found a significant increase in caspase-3 expression and significant decrease in Bcl-2, Ki67, and VEGFR-2 expression in the high-dose group. Both doses demonstrated significant positive effects on plasma lipid metabolism in rats. F7 significantly decreased survival of MCF-7 cells in vitro in MTT assay by dose- and time-dependent manner compared to control. F7 prevented cell cycle progression by significant enrichment in G1 cell populations. Incubation with F7 showed significant increase in the percentage of annexin V-/PI-positive MCF-7 cells and DNA fragmentation. CONCLUSIONS: Our results reveal a substantial tumour-suppressive effect of F7 in the breast cancer model. We propose that the effects of phytochemicals present in this fruit extract are responsible for observed potent anti-cancer activities.
Department of Anatomy Comenius University Martin Slovakia
Department of Histology and Embryology Comenius University Martin Slovakia
Department of Medical Biochemistry Comenius University Martin Slovakia
See more in PubMed
Eur J Cancer Prev. 2014 Nov;23(6):506-13 PubMed
Colloids Surf B Biointerfaces. 2014 Jan 1;113:15-24 PubMed
Oncol Rep. 2013 Nov;30(5):2357-64 PubMed
Asian Pac J Trop Med. 2013 Dec;6(12):925-33 PubMed
Am J Epidemiol. 1999 May 15;149(10):943-9 PubMed
Cancer Epidemiol Biomarkers Prev. 2007 Nov;16(11):2285-92 PubMed
Biochem Pharmacol. 2007 Aug 15;74(4):533-44 PubMed
Eur J Cancer Prev. 2014 Mar;23(2):84-9 PubMed
PLoS One. 2013 Dec 16;8(12):e82288 PubMed
Eur J Cancer Prev. 2012 Mar;21(2):163-70 PubMed
Int J Cancer. 2008 Aug 1;123(3):664-71 PubMed
Acta Biochim Biophys Sin (Shanghai). 2014 Jun;46(6):460-70 PubMed
Cancer Lett. 2014 Oct 10;353(1):95-103 PubMed
J Immunol Methods. 1983 Dec 16;65(1-2):55-63 PubMed
Breast. 2014 Jun;23(3):259-63 PubMed
Amino Acids. 2003 Dec;25(3-4):233-47 PubMed
JAMA. 2002 Nov 27;288(20):2569-78 PubMed
Physiol Res. 2008;57(3):413-20 PubMed
PLoS One. 2013 Jul 05;8(7):e68566 PubMed
Nutr Cancer. 2014;66(7):1237-46 PubMed
Environ Toxicol Pharmacol. 2014 Jan;37(1):234-47 PubMed
Int J Oncol. 2014 Feb;44(2):473-80 PubMed
Nutrition. 2015 Apr;31(4):560-9 PubMed
Nutr Cancer. 2004;50(1):1-7 PubMed
J Nutr. 2004 Jan;134(1):5-10 PubMed
Phytother Res. 2013 Mar;27(3):397-403 PubMed
Am J Chin Med. 2014;42(2):465-83 PubMed
Am J Clin Nutr. 1994 May;59(5 Suppl):1162S-1165S PubMed
J Mammary Gland Biol Neoplasia. 2000 Apr;5(2):187-200 PubMed
Gen Physiol Biophys. 2001 Dec;20(4):431-8 PubMed
Nat Rev Cancer. 2003 Apr;3(4):276-85 PubMed
Salvia officinalis L. exerts oncostatic effects in rodent and in vitro models of breast carcinoma
Anticancer Activities of Thymus vulgaris L. in Experimental Breast Carcinoma in Vivo and in Vitro
Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma
Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model
