SlDREB2, a tomato dehydration-responsive element-binding 2 transcription factor, mediates salt stress tolerance in tomato and Arabidopsis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26082265
DOI
10.1111/pce.12591
Knihovny.cz E-zdroje
- Klíčová slova
- Arabidopsis thaliana, DREB2, Solanum lycopersicum, salinity tolerance.,
- MeSH
- Arabidopsis genetika fyziologie MeSH
- chlorid sodný farmakologie MeSH
- dehydratace MeSH
- fyziologický stres MeSH
- geneticky modifikované rostliny MeSH
- kořeny rostlin genetika fyziologie MeSH
- kyselina abscisová farmakologie MeSH
- molekulární sekvence - údaje MeSH
- mutace MeSH
- období sucha MeSH
- regulace genové exprese u rostlin * MeSH
- regulátory růstu rostlin farmakologie MeSH
- rostlinné proteiny genetika metabolismus MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- semenáček genetika fyziologie MeSH
- Solanum lycopersicum genetika fyziologie MeSH
- stanovení celkové genové exprese MeSH
- tolerance k soli MeSH
- transkripční faktory genetika metabolismus MeSH
- transkriptom * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chlorid sodný MeSH
- kyselina abscisová MeSH
- regulátory růstu rostlin MeSH
- rostlinné proteiny MeSH
- transkripční faktory MeSH
To counter environmental cues, cultivated tomato (Solanum lycopersicum L.) has evolved adaptive mechanisms requiring regulation of downstream genes. The dehydration-responsive element-binding protein 2 (DREB2) transcription factors regulate abiotic stresses responses in plants. Herein, we isolated a novel DREB2-type regulator involved in salinity response, named SlDREB2. Spatio-temporal expression profile together with investigation of its promoter activity indicated that SlDREB2 is expressed during early stages of seedling establishment and in various vegetative and reproductive organs of adult plants. SlDREB2 is up-regulated in roots and young leaves following exposure to NaCl, but is also induced by KCl and drought. Its overexpression in WT Arabidopsis and atdreb2a mutants improved seed germination and plant growth in presence of different osmotica. In tomato, SlDREB2 affected vegetative and reproductive organs development and the intronic sequence present in the 5' UTR drives its expression. Physiological, biochemical and transcriptomic analyses showed that SlDREB2 enhanced plant tolerance to salinity by improvement of K(+) /Na(+) ratio, and proline and polyamines biosynthesis. Exogenous hormonal treatments (abscisic acid, auxin and cytokinins) and analysis of WT and 35S::SlDREB2 tomatoes hormonal contents highlighted SlDREB2 involvement in abscisic acid biosynthesis/signalling. Altogether, our results provide an overview of SlDREB2 mode of action during early salt stress response.
Département Sciences du vivant Centre wallon de Recherches Agronomiques B 5030 Gembloux Belgium
Groupe de Recherche en Physiologie Végétale B 1348 Louvain la Neuve Belgium
Institut des Sciences de la Vie B 1348 Louvain la Neuve Belgium
Citace poskytuje Crossref.org
GENBANK
AAN77051, ABV03750, ADE35085, ADJ67432, ADZ15315, AF092655, AFU35562, AFU35563, AGI78251, AI491005, AI896522, AJ271093, AJ785329, AK247308, AK247408, AK319251, AK319263, AK319264, AK319894, AK320589, AK320933, AK322126, AK322252, AK324617, AK324676, AK325041, AK325517, AK326408, AK327173, AK327683, AK328035, AK328092, AK328302, AK329161, AK329281, AK330114, AW041529, AW217177, AW624869, AW648744, BF096555, BF096870, BM412776, BP879017, BT013336, CAB93939, DB706854, DB718607, DQ335097, DY523801, EF091574, EG553122, EG553756, EU670750, GO374968, GSE64010, HQ698902, U37840, X14449
