SlDREB2, a tomato dehydration-responsive element-binding 2 transcription factor, mediates salt stress tolerance in tomato and Arabidopsis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26082265
DOI
10.1111/pce.12591
Knihovny.cz E-resources
- Keywords
- Arabidopsis thaliana, DREB2, Solanum lycopersicum, salinity tolerance.,
- MeSH
- Arabidopsis genetics physiology MeSH
- Sodium Chloride pharmacology MeSH
- Dehydration MeSH
- Stress, Physiological MeSH
- Plants, Genetically Modified MeSH
- Plant Roots genetics physiology MeSH
- Abscisic Acid pharmacology MeSH
- Molecular Sequence Data MeSH
- Mutation MeSH
- Droughts MeSH
- Gene Expression Regulation, Plant * MeSH
- Plant Growth Regulators pharmacology MeSH
- Plant Proteins genetics metabolism MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Seedlings genetics physiology MeSH
- Solanum lycopersicum genetics physiology MeSH
- Gene Expression Profiling MeSH
- Salt Tolerance MeSH
- Transcription Factors genetics metabolism MeSH
- Transcriptome * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Sodium Chloride MeSH
- Abscisic Acid MeSH
- Plant Growth Regulators MeSH
- Plant Proteins MeSH
- Transcription Factors MeSH
To counter environmental cues, cultivated tomato (Solanum lycopersicum L.) has evolved adaptive mechanisms requiring regulation of downstream genes. The dehydration-responsive element-binding protein 2 (DREB2) transcription factors regulate abiotic stresses responses in plants. Herein, we isolated a novel DREB2-type regulator involved in salinity response, named SlDREB2. Spatio-temporal expression profile together with investigation of its promoter activity indicated that SlDREB2 is expressed during early stages of seedling establishment and in various vegetative and reproductive organs of adult plants. SlDREB2 is up-regulated in roots and young leaves following exposure to NaCl, but is also induced by KCl and drought. Its overexpression in WT Arabidopsis and atdreb2a mutants improved seed germination and plant growth in presence of different osmotica. In tomato, SlDREB2 affected vegetative and reproductive organs development and the intronic sequence present in the 5' UTR drives its expression. Physiological, biochemical and transcriptomic analyses showed that SlDREB2 enhanced plant tolerance to salinity by improvement of K(+) /Na(+) ratio, and proline and polyamines biosynthesis. Exogenous hormonal treatments (abscisic acid, auxin and cytokinins) and analysis of WT and 35S::SlDREB2 tomatoes hormonal contents highlighted SlDREB2 involvement in abscisic acid biosynthesis/signalling. Altogether, our results provide an overview of SlDREB2 mode of action during early salt stress response.
Département Sciences du vivant Centre wallon de Recherches Agronomiques B 5030 Gembloux Belgium
Groupe de Recherche en Physiologie Végétale B 1348 Louvain la Neuve Belgium
Institut des Sciences de la Vie B 1348 Louvain la Neuve Belgium
References provided by Crossref.org
GENBANK
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