Markers of oxidative stress and antioxidant status in relation to inflammatory mediators in septic patients (SPs) during the course of sepsis and after recovery were analysed. Patients were 30 critically ill adults in severe sepsis/septic shock, 19 of which completed 3 samplings (S1: within 24 h after onset of sepsis, S7: 7 days after S1, R7: 7 days after clinical recovery). Comparing SPs with healthy controls (HCs), enhanced C-reactive protein, procalcitonin, bilirubin and CuZn-superoxide dismutase activity were found at S1 only. Oxidized low-density lipoprotein, conjugated dienes and nitrotyrosine were increased at S1, culminated at S7 and reverted nearly to HC levels at R7. Reduced catalase activity and serum amyloid were observed at S1 and endured until R7. Increase in IL-6, IL-10 and tumour necrosis factor alpha (TNF-α) with accompanying decrease in apolipoprotein A1, high-density lipoprotein (HDL) cholesterol, selenium, zinc, albumin, paraoxonase 1 and glutathione peroxidase 1 activity appeared at S1 and persisted until R7. TNF-α, IL-10 and markers of oxidative stress were in negative correlation with HDL cholesterol and albumin at R7. After clinical recovery, increased cytokines and decreased antioxidants were accompanied by lower albumin and HDL cholesterol levels. During this important and beneficial period of tissue repair, patients with prolonged persistence of this status are probably more vulnerable to secondary infections and should be dealt with as constituting a high-risk population.

4th Department of Internal Medicine 1st Faculty of Medicine and General Teaching Hospital Charles University Prague U Nemocnice 2 128 08 Prague Czech Republic

Department of Cell Biology Faculty of Science Charles University Prague Viničná 7 128 44 Prague Czech Republic

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J Crit Care. 1995 Sep;10(3):122-35 PubMed

Biochem Pharmacol. 2000 Jan 15;59(2):187-94 PubMed

Clin Sci (Lond). 2009 Jan;116(2):87-98 PubMed

Crit Care Med. 2008 Jan;36(1):296-327 PubMed

Injury. 2011 Jul;42(7):617-29 PubMed

Arch Surg. 2001 Oct;136(10):1201-7 PubMed

Clin Chem. 1995 Jun;41(6 Pt 1):867-71 PubMed

Intensive Care Med. 1991;17(1):40-4 PubMed

Pharmacol Ther. 2013 Mar;137(3):341-51 PubMed

Crit Care Clin. 2006 Jul;22(3):489-501, ix PubMed

Metabolism. 1988 Sep;37(9):859-65 PubMed

Nature. 2014 Jun 5;510(7503):92-101 PubMed

J Biol Chem. 1998 Mar 13;273(11):6088-95 PubMed

Crit Care Med. 2003 Apr;31(4):1250-6 PubMed

Clin Chim Acta. 1998 Jun 22;274(2):177-88 PubMed

Arch Surg. 1999 Jan;134(1):36-42 PubMed

J Biol Chem. 1957 Nov;229(1):189-97 PubMed

Curr Opin Infect Dis. 2012 Jun;25(3):328-36 PubMed

Free Radic Biol Med. 2006 Feb 1;40(3):398-406 PubMed

Adv Exp Med Biol. 2010;660:89-97 PubMed

Clin Exp Med. 2010 Mar;10(1):21-5 PubMed

Am J Hum Genet. 1983 Nov;35(6):1126-38 PubMed

Biochem J. 1998 May 15;332 ( Pt 1):251-5 PubMed

Surg Infect (Larchmt). 2009 Aug;10(4):339-48 PubMed

J Crit Care. 2010 Jun;25(2):361.e7-361.e12 PubMed

Crit Care Med. 1995 Apr;23(4):646-51 PubMed

Circulation. 2009 Mar 3;119(8):1135-45 PubMed

Free Radic Biol Med. 1999 Apr;26(7-8):892-904 PubMed

Indian J Med Res. 2007 Feb;125(2):143-8 PubMed

Crit Care Med. 2003 May;31(5):1359-66 PubMed

Clin Biochem. 2009 Sep;42(13-14):1368-74 PubMed

Crit Care Med. 2000 Aug;28(8):2779-85 PubMed

Front Public Health. 2013 Nov 15;1:51 PubMed

Crit Care Med. 2001 Jul;29(7):1303-10 PubMed

Crit Care. 2007;11(2):R49 PubMed

J Antimicrob Chemother. 2011 Apr;66 Suppl 2:ii33-40 PubMed

N Engl J Med. 2003 Jan 9;348(2):138-50 PubMed

N Engl J Med. 1969 Nov 13;281(20):1081-6 PubMed

Biofactors. 2008;33(2):107-19 PubMed

Mediators Inflamm. 2008;2008:168652 PubMed

Clin Chem. 1997 Apr;43(4):562-8 PubMed

Biosci Rep. 2011 Feb;31(1):63-76 PubMed

J Crit Care. 2008 Dec;23 (4):537-41 PubMed

Saudi J Gastroenterol. 2010 Apr-Jun;16(2):79-83 PubMed

Arch Intern Med. 2007 Aug 13-27;167(15):1655-63 PubMed

Pediatr Clin North Am. 2008 Jun;55(3):647-68, xi PubMed

Crit Care Med. 2001 Aug;29(8):1563-8 PubMed

Am J Respir Crit Care Med. 2008 Jun 1;177(11):1242-7 PubMed

Clin Biochem. 1996 Apr;29(2):139-44 PubMed

Am Rev Respir Dis. 1992 Oct;146(4):985-9 PubMed

Biochim Biophys Acta. 2013 Dec;1830(12):5405-17 PubMed

J Clin Pathol. 2001 Mar;54(3):176-86 PubMed

Eur J Clin Invest. 2007 Jul;37(7):573-9 PubMed

Atherosclerosis. 2006 Jun;186(2):396-401 PubMed

JAMA. 2001 Oct 10;286(14):1754-8 PubMed

Crit Care Med. 1998 Sep;26(9):1536-44 PubMed

Crit Care Med. 1999 Jul;27(7):1262-4 PubMed

J Infect Dis. 2000 Jan;181(1):176-80 PubMed

Free Radic Biol Med. 2004 Dec 15;37(12):1986-94 PubMed

N Engl J Med. 1999 Feb 11;340(6):448-54 PubMed

Aliment Pharmacol Ther. 2002 Dec;16 Suppl 5:6-11 PubMed

J Surg Res. 1994 Jul;57(1):197-204 PubMed

Clin Chest Med. 2008 Dec;29(4):617-25, viii PubMed

Crit Care. 2010;14(1):R15 PubMed

Atherosclerosis. 2011 Jan;214(1):20-36 PubMed

High Dose Fish Oil Added to Various Lipid Emulsions Normalizes Superoxide Dismutase 1 Activity in Home Parenteral Nutrition Patients

Plasma Phospholipid Fatty Acid Profile is Altered in Both Septic and Non-Septic Critically Ill: A Correlation with Inflammatory Markers and Albumin