Structure, mechanical characteristics and in vitro degradation, cytotoxicity, genotoxicity and mutagenicity of novel biodegradable Zn-Mg alloys
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26478283
DOI
10.1016/j.msec.2015.08.015
PII: S0928-4931(15)30271-X
Knihovny.cz E-resources
- Keywords
- Cytotoxicity, Genotoxicity, In vitro testing, Mechanical properties, Zinc,
- MeSH
- Biocompatible Materials chemistry toxicity MeSH
- Magnesium chemistry toxicity MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- DNA Damage drug effects MeSH
- Alloys chemistry toxicity MeSH
- Cell Survival drug effects MeSH
- Zinc chemistry toxicity MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Magnesium MeSH
- Alloys MeSH
- Zinc MeSH
Zn-(0-1.6)Mg (in wt.%) alloys were prepared by hot extrusion at 300 °C. The structure, mechanical properties and in vitro biocompatibility of the alloys were investigated. The hot-extruded magnesium-based WE43 alloy was used as a control. Mechanical properties were evaluated by hardness, compressive and tensile testing. The cytotoxicity, genotoxicity (comet assay) and mutagenicity (Ames test) of the alloy extracts and ZnCl2 solutions were evaluated with the use of murine fibroblasts L929 and human osteosarcoma cell line U-2 OS. The microstructure of the Zn alloys consisted of recrystallized Zn grains of 12 μm in size and fine Mg2Zn11 particles arranged parallel to the hot extrusion direction. Mechanical tests revealed that the hardness and strength increased with increasing Mg concentration. The Zn-0.8 Mg alloys showed the best combination of tensile mechanical properties (tensile yield strength of 203 MPa, ultimate tensile strength of 301 MPa and elongation of 15%). At higher Mg concentrations the plasticity of Zn-Mg alloys was deteriorated. Cytotoxicity tests with alloy extracts and ZnCl2 solutions proved the maximum safe Zn(2+) concentrations of 120 μM and 80 μM for the U-2 OS and L929 cell lines, respectively. Ames test with extracts of alloys indicated that the extracts were not mutagenic. The comet assay demonstrated that 1-day extracts of alloys were not genotoxic for U-2 OS and L929 cell lines after 1-day incubation.
References provided by Crossref.org
Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials
Zn-Mg Biodegradable Composite: Novel Material with Tailored Mechanical and Corrosion Properties
The Fundamental Comparison of Zn-2Mg and Mg-4Y-3RE Alloys as a Perspective Biodegradable Materials
