Transgelin is upregulated in stromal cells of lymph node positive breast cancer
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26639304
DOI
10.1016/j.jprot.2015.11.025
PII: S1874-3919(15)30198-6
Knihovny.cz E-resources
- Keywords
- Breast cancer, Lymph node metastasis, Proteomics, Transgelin, Transgelin-2,
- MeSH
- Stromal Cells metabolism pathology MeSH
- Humans MeSH
- Lymph Nodes metabolism pathology MeSH
- Microfilament Proteins metabolism MeSH
- Biomarkers, Tumor metabolism MeSH
- Neoplasm Proteins metabolism MeSH
- Breast Neoplasms metabolism pathology MeSH
- Pilot Projects MeSH
- Gene Expression Regulation, Neoplastic MeSH
- Neoplasm Grading MeSH
- Muscle Proteins metabolism MeSH
- Up-Regulation MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Microfilament Proteins MeSH
- Biomarkers, Tumor MeSH
- Neoplasm Proteins MeSH
- Muscle Proteins MeSH
- transgelin MeSH Browser
UNLABELLED: Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients. We further clinically validated 2-DE results in an independent cohort of 48 low grade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC-MS/MS, mTRAQ-SRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed significant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the second cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors. BIOLOGICAL SIGNIFICANCE: Both transgelin and transgelin-2 have been previously described as potential markers of many types of cancer. We are specifying this connection to metastatic affection of lymph nodes and cell differentiation in breast cancer. In the wider context, the results of our study highlight tumor stroma as a source of cancer biomarkers and point out how measured levels of tissue markers can actually reflect cellular feature of cancer mass.
Department of Biochemistry Faculty of Science Masaryk University Brno Czech Republic
Masaryk Memorial Cancer Institute Regional Centre for Applied Molecular Oncology Brno Czech Republic
References provided by Crossref.org
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