The effect of alpha-tocopheryl succinate on succinate respiration in rat liver mitochondria
Language English Country Czech Republic Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
26674283
DOI
10.33549/physiolres.933219
PII: 933519
Knihovny.cz E-resources
- MeSH
- alpha-Tocopherol metabolism pharmacology MeSH
- Cell Membrane metabolism MeSH
- Cell Respiration drug effects MeSH
- Time Factors MeSH
- Energy Metabolism drug effects MeSH
- Hepatocytes drug effects metabolism MeSH
- Mitochondria, Liver drug effects metabolism MeSH
- Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone pharmacology MeSH
- Oxidative Phosphorylation drug effects MeSH
- Cell Membrane Permeability MeSH
- Rats, Wistar MeSH
- Electron Transport Complex II metabolism MeSH
- Uncoupling Agents pharmacology MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- alpha-Tocopherol MeSH
- Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone MeSH
- Electron Transport Complex II MeSH
- Uncoupling Agents MeSH
We compared the effect of alpha-tocopheryl succinate (TOS) on succinate-dependent respiration in rat liver mitochondria, homogenate and permeabilized hepatocytes in both a coupled and uncoupled state. In isolated mitochondria, a significant inhibitory effect was observed at a concentration of 5 microM, in liver homogenate at 25 microM and in permeabilized hepatocytes at 50 microM. The inhibitory effect of TOS on succinate respiration in an uncoupled state was less pronounced than in a coupled state in all the experimental models tested. When the concentration dependence of the TOS inhibitory effect was tested, the most sensitive in both states were isolated mitochondria; the most resistant were permeabilized hepatocytes.
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