Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
26725215
DOI
10.1016/j.bios.2015.12.010
PII: S0956-5663(15)30661-8
Knihovny.cz E-resources
- Keywords
- Dehydrochlorinase, Environmental monitoring, Field-testing, Haloalkane dehalogenase, Halogenated pollutant, Optical biosensor,
- MeSH
- Bacteria enzymology MeSH
- Biosensing Techniques instrumentation MeSH
- Water Pollutants, Chemical analysis metabolism MeSH
- Hydrocarbons, Chlorinated analysis metabolism MeSH
- Fluorescence MeSH
- Spectrometry, Fluorescence instrumentation MeSH
- Hydrolases metabolism MeSH
- Environmental Monitoring instrumentation MeSH
- Water analysis MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Water Pollutants, Chemical MeSH
- Hydrocarbons, Chlorinated MeSH
- haloalkane dehalogenase MeSH Browser
- Hydrolases MeSH
- Water MeSH
An advanced optical biosensor was developed based on the enzymatic reaction with halogenated aliphatic hydrocarbons that is accompanied by the fluorescence change of pH indicator. The device is applicable for the detection of halogenated contaminants in water samples with pH ranging from 4 to 10 and temperature ranging from 5 to 60°C. Main advantages of the developed biosensor are small size (60×30×190mm(3)) and portability, which together with short measurement time of 1min belong to crucial attributes of analytical technique useful for routine environmental monitoring. The biosensor was successfully applied for the detection of several important halogenated pollutants under laboratory conditions, e.g., 1,2-dichloroethane, 1,2,3-trichloropropane and γ-hexachlorocyclohexane, with the limits of detection of 2.7, 1.4 and 12.1mgL(-1), respectively. The continuous monitoring was demonstrated by repetitive injection of halogenated compound into measurement solution. Consequently, field trials under environmental settings were performed. The presence of 1,2-dichloroethane (10mgL(-1)) was proved unambiguously on one of three potentially contaminated sites in Czech Republic, and the same contaminant was monitored on contaminated locality in Serbia. Equipped by Global Positioning System, the biosensor was used for creation of a precise map of contamination. Concentrations determined by biosensor and by gas chromatograph coupled with mass spectrometer exhibited the correlation coefficient of 0.92, providing a good confidence for the routine use of the biosensor system in both field screening and monitoring.
References provided by Crossref.org
Sensitive operation of enzyme-based biodevices by advanced signal processing
