Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27086677
DOI
10.1002/jbm.b.33663
Knihovny.cz E-resources
- Keywords
- hyaluronic acid, phospholipid, protein, total hip and knee replacement, viscosity,
- MeSH
- Biomimetic Materials chemistry MeSH
- Adult MeSH
- Phospholipids analysis metabolism MeSH
- gamma-Globins analysis metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Lubricants chemistry MeSH
- Arthroplasty, Replacement, Hip MeSH
- Aged MeSH
- Synovial Fluid chemistry metabolism MeSH
- Arthroplasty, Replacement, Knee MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phospholipids MeSH
- gamma-Globins MeSH
- Lubricants MeSH
Wear testing of total joint replacement (TJR) is mandatory in preclinical testing before implantation of TJR into the human body. Testing is governed by current international standards that recommend bovine serum (BS) as a lubricating fluid to replace synovial fluid (SF). Recently, the use of BS has been criticized because of differences in content, fluid characteristics, and nonhuman origin. As a result, a more realistic lubricant mimicking SF is needed. To define SF composition, we analyzed SF obtained during revisions of total hip and knee arthroplasties and compared it with SF obtained during primary arthroplasties and from patients without TJR. Samples were acquired from 152 patients. We found that the median total protein concentration for all SF was 36.8 mg/mL, which is significantly higher than concentrations currently recommended by the ISO standards. The γ-globulin concentration was significantly higher and the phospholipid concentration significantly lower in patients with revision of TJR compared with patients without TJR. No significant difference was found in hyaluronic acid concentration and viscosity among the groups. Our results support the need to improve the definition of a more clinically relevant wear testing lubricant in the ISO standards. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1422-1431, 2017.
References provided by Crossref.org
Analysis of Chemisorbed Tribo-Film for Ceramic-on-Ceramic Hip Joint Prostheses by Raman Spectroscopy
