Chromosome identification for the carnivorous plant Genlisea margaretae
Language English Country Austria Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27153834
DOI
10.1007/s00412-016-0599-0
PII: 10.1007/s00412-016-0599-0
Knihovny.cz E-resources
- Keywords
- BACs, Genlisea, Karyotyping, Multicolor fluorescence in situ hybridization (mcFISH), Reprobing,
- MeSH
- Chromosomes, Plant genetics metabolism MeSH
- Genome Size MeSH
- Genome, Plant MeSH
- In Situ Hybridization, Fluorescence MeSH
- Magnoliopsida genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.
Leibniz Institute of Plant Genetics and Crop Plant Research 06466 Gatersleben Stadt Seeland Germany
Plant Resource Center Vietnam Academy of Agricultural Science Ankhanh Hoaiduc Hanoi Vietnam
See more in PubMed
Zoolog Sci. 2009 Mar;26(3):187-90 PubMed
New Phytol. 2016 Jan;209(1):354-63 PubMed
Plant Biol (Stuttg). 2004 Jul;6(4):477-90 PubMed
Plant J. 2006 Sep;47(6):977-86 PubMed
Chromosome Res. 2003;11(3):195-204 PubMed
Genetics. 2006 May;173(1):349-62 PubMed
Front Plant Sci. 2015 Aug 20;6:613 PubMed
Plant J. 2014 Jan;77(1):16-30 PubMed
PLoS One. 2014 Dec 10;9(12 ):e115108 PubMed
Mol Phylogenet Evol. 2010 Aug;56(2):768-83 PubMed
Methods Mol Biol. 2006;323:173-86 PubMed
Ann Bot. 2003 Apr;91(5):547-57 PubMed
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5224-9 PubMed
Plant Cell. 2008 Oct;20(10):2559-70 PubMed
Front Microbiol. 2015 Jul 14;6:526 PubMed
Genetics. 2011 Jan;187(1):37-49 PubMed
Plant J. 2000 Jul;23(2):233-43 PubMed
Curr Opin Plant Biol. 2016 Apr;30:108-15 PubMed
Theor Appl Genet. 2005 Nov;111(8):1617-22 PubMed
Ann Bot. 2006 Feb;97(2):205-16 PubMed
Plant Cell. 2010 Jul;22(7):2277-90 PubMed
Genetics. 2008 Feb;178(2):1117-22 PubMed
Genome. 2005 Apr;48(2):341-6 PubMed
Plant Biol (Stuttg). 2006 Nov;8(6):770-7 PubMed
Ann Bot. 2014 Dec;114(8):1651-63 PubMed
Nat Protoc. 2012 Feb 16;7(3):479-99 PubMed
Plant J. 2015 Dec;84(6):1087-99 PubMed
