Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27266660
DOI
10.1016/j.bios.2016.05.073
PII: S0956-5663(16)30503-6
Knihovny.cz E-zdroje
- Klíčová slova
- Cardiomyocyte contraction, Drug testing, Human stem cell, Micromechanical biosensor,
- MeSH
- agonisté adrenergních beta-receptorů farmakologie MeSH
- antagonisté beta-1-adrenergních receptorů farmakologie MeSH
- biomechanika účinky léků MeSH
- biosenzitivní techniky přístrojové vybavení metody MeSH
- buněčné kultury přístrojové vybavení metody MeSH
- buněčné linie MeSH
- design vybavení MeSH
- isoprenalin farmakologie MeSH
- kardiomyocyty cytologie účinky léků metabolismus MeSH
- kontrakce myokardu účinky léků MeSH
- lidé MeSH
- metoprolol farmakologie MeSH
- mikroskopie atomárních sil přístrojové vybavení metody MeSH
- pluripotentní kmenové buňky cytologie MeSH
- preklinické hodnocení léčiv přístrojové vybavení metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- agonisté adrenergních beta-receptorů MeSH
- antagonisté beta-1-adrenergních receptorů MeSH
- isoprenalin MeSH
- metoprolol MeSH
Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.
CEITEC Masaryk University Brno Czech Republic
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
PhyMedExp INSERM U1046 University of Montpellier Montpellier France
Citace poskytuje Crossref.org
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DMD Pluripotent Stem Cell Derived Cardiac Cells Recapitulate in vitro Human Cardiac Pathophysiology
