Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27266660
DOI
10.1016/j.bios.2016.05.073
PII: S0956-5663(16)30503-6
Knihovny.cz E-resources
- Keywords
- Cardiomyocyte contraction, Drug testing, Human stem cell, Micromechanical biosensor,
- MeSH
- Adrenergic beta-Agonists pharmacology MeSH
- Adrenergic beta-1 Receptor Antagonists pharmacology MeSH
- Biomechanical Phenomena drug effects MeSH
- Biosensing Techniques instrumentation methods MeSH
- Cell Culture Techniques instrumentation methods MeSH
- Cell Line MeSH
- Equipment Design MeSH
- Isoproterenol pharmacology MeSH
- Myocytes, Cardiac cytology drug effects metabolism MeSH
- Myocardial Contraction drug effects MeSH
- Humans MeSH
- Metoprolol pharmacology MeSH
- Microscopy, Atomic Force instrumentation methods MeSH
- Pluripotent Stem Cells cytology MeSH
- Drug Evaluation, Preclinical instrumentation methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adrenergic beta-Agonists MeSH
- Adrenergic beta-1 Receptor Antagonists MeSH
- Isoproterenol MeSH
- Metoprolol MeSH
Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.
CEITEC Masaryk University Brno Czech Republic
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
PhyMedExp INSERM U1046 University of Montpellier Montpellier France
References provided by Crossref.org
Salbutamol attenuates arrhythmogenic effect of aminophylline in a hPSC-derived cardiac model
DMD Pluripotent Stem Cell Derived Cardiac Cells Recapitulate in vitro Human Cardiac Pathophysiology
