Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Cell cycle synchronization, Cytogenetic mapping, Fluorochrome, Low-copy probe, Mitotic metaphase chromosomes, Post-fixation, Probe purification, Quantum yield,
- MeSH
- Chromosomes, Plant * MeSH
- DNA, Plant genetics MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Hordeum genetics MeSH
- Chromosome Mapping methods MeSH
- Mitosis MeSH
- DNA Copy Number Variations * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Plant MeSH
Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on mitotic and meiotic chromosomes and interphase nuclei. It was developed in early 1980s and since then it has contributed to numerous studies and important discoveries. Over the decades, the protocol was modified for ease of use, allowing for localizing multiple probes simultaneously and increasing its sensitivity and specificity. Despite the continuous improvements, the ability to detect short single-copy sequences of only a few kilobases or less, such as genes, remains limited. Here, we provide a detailed protocol for detection of short, single- or low-copy sequences on plant mitotic metaphase chromosomes.
References provided by Crossref.org
Advances in the Molecular Cytogenetics of Bananas, Family Musaceae
The Dark Matter of Large Cereal Genomes: Long Tandem Repeats
