Analytical Determination of Auxins and Cytokinins
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Auxin, Cytokinins, Liquid chromatography, Mass spectrometry, Phytohormones,
- MeSH
- Chromatography, Liquid MeSH
- Cytokinins analysis chemistry isolation & purification MeSH
- Indoleacetic Acids analysis chemistry isolation & purification MeSH
- Metabolomics methods MeSH
- Plant Growth Regulators analysis MeSH
- Plant Extracts analysis chemistry isolation & purification MeSH
- Tandem Mass Spectrometry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokinins MeSH
- Indoleacetic Acids MeSH
- Plant Growth Regulators MeSH
- Plant Extracts MeSH
Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures.
References provided by Crossref.org
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