A recently presented taxonomical arrangement of the moss genus Orthotrichum Hedw. s.l. substantially changed the traditional view of the taxon that had been accepted throughout the twentieth century. This paper provides the results of mitogenomic studies that strongly support the new taxonomical concept. Comparative analyses presented in this study confirmed the stable structure of moss mitogenomes. Moreover, 17 complete mitogenome sequences were used to identify the major evolutionary groups, including 11 newly sequenced ones, for this study. The analysis of mitochondrial hotspots revealed intron 4 of the cox1 gene to be the most variable non-coding region. The most variable protein-coding genes in the tribe Orthotricheae were ccmFC and tatC. The intergenic and intronic hotspots of Orthotrichum s.l. identified in the present study do not correspond to those described in vascular plant mitogenomes.

Department of Biology and Ecology University of Ostrava Chittussiho 10 710 00 Ostrava Czech Republic

Department of Botany and Nature Protection University of Warmia and Mazury Plac Łódzki 1 10 727 Olsztyn Poland

Laboratory of Bryology Institute of Botany Polish Academy of Sciences Lubicz 46 31 512 Kraków Poland

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