First description in Europe of the emergence of Enterococcus faecium ST117 carrying both vanA and vanB genes, isolated in Greece
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28754459
DOI
10.1016/j.jgar.2017.07.010
PII: S2213-7165(17)30138-8
Knihovny.cz E-resources
- Keywords
- Enterococcus faecium, ST117, vanA, vanB,
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Genes, Bacterial genetics MeSH
- Bacterial Proteins genetics MeSH
- Bacteremia microbiology MeSH
- Enterococcus faecium drug effects genetics isolation & purification pathogenicity MeSH
- Vancomycin-Resistant Enterococci genetics MeSH
- Phenotype MeSH
- Genotype MeSH
- Gram-Positive Bacterial Infections microbiology MeSH
- Humans MeSH
- Carbon-Oxygen Ligases genetics MeSH
- Microbial Sensitivity Tests MeSH
- Molecular Epidemiology * MeSH
- Multilocus Sequence Typing MeSH
- Hospitals MeSH
- Plasmids genetics MeSH
- Protein Kinases genetics MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Gene Expression Regulation, Bacterial MeSH
- Vancomycin Resistance MeSH
- Teicoplanin pharmacology MeSH
- Transcription Factors genetics MeSH
- DNA Transposable Elements MeSH
- Vancomycin pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Europe MeSH
- Greece epidemiology MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- Carbon-Oxygen Ligases MeSH
- Protein Kinases MeSH
- Teicoplanin MeSH
- Transcription Factors MeSH
- DNA Transposable Elements MeSH
- VanA ligase, Bacteria MeSH Browser
- VanB protein, Enterococcus MeSH Browser
- Vancomycin MeSH
- VanS protein, Enterococcus MeSH Browser
- VanZ protein, Enterococcus faecium MeSH Browser
OBJECTIVES: An Enterococcus faecium isolate (Efa-125) carrying both the vanA and vanB genes was recovered from a patient with bacteraemia treated in a Greek hospital. Since this is the first description in Europe of E. faecium carrying both vanA and vanB genes, the isolate was further studied. METHODS: Susceptibility to several antibiotics was determined using the VITEK®2 automated system. The isolate was typed by multilocus sequence typing (MLST). To define the genetic units of the vanA and vanB genes, the plasmid content of Efa-125 was analysed by pulsed-field gel electrophoresis (PFGE) of total DNA digested with S1 nuclease followed by hybridisation with digoxigenin-labelled vanA and vanB probes. In addition, plasmids and chromosomes were sequenced using the Illumina MiSeq platform. RESULTS: E. faecium Efa-125 belonged to ST117 and expressed resistance both to vancomycin and teicoplanin, with minimum inhibitory concentrations (MICs) for both of 256mg/L. The vanA gene was carried on a 29 320-bp plasmid exhibiting high similarity to pA6981 previously characterised from Enterococcus gallinarum A6981, whereas vanB was part of a Tn1549-like transposon integrated into the chromosome. Expression of the VanA phenotype was correlated with the presence of intact vanZ and vanS genes. CONCLUSIONS: This is the first detection in Greece of vanA-vanB genotype/VanA phenotype E. faecium and indicates an evolving epidemiology of vancomycin-resistant enterococci.
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