Effect of Piper betle on Giardia intestinalis infection in vivo
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29154846
DOI
10.1016/j.exppara.2017.11.005
PII: S0014-4894(17)30236-9
Knihovny.cz E-zdroje
- Klíčová slova
- Antigiardial activity, Drug of choice, Giardia intestinalis, Natural antiparasitics, Parasites, Piper betle,
- MeSH
- antiprotozoální látky farmakologie terapeutické užití MeSH
- feces parazitologie MeSH
- Gerbillinae MeSH
- Giardia lamblia účinky léků ultrastruktura MeSH
- giardiáza farmakoterapie MeSH
- lineární modely MeSH
- listy rostlin chemie MeSH
- lyofilizace MeSH
- metronidazol farmakologie terapeutické užití MeSH
- mikroskopie elektronová rastrovací MeSH
- Piper betle chemie MeSH
- průjem farmakoterapie parazitologie MeSH
- rostlinné extrakty farmakologie terapeutické užití MeSH
- tenké střevo parazitologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indonésie MeSH
- Názvy látek
- antiprotozoální látky MeSH
- metronidazol MeSH
- rostlinné extrakty MeSH
Piper betle has been used as a medicinal plant in traditional medical systems throughout South and South East Asia. Experimental studies have revealed its wide and diverse biological and pharmacological effects. In this study, antigiardial activity of Piper betle was tested using experimental infections of Giardia intestinalis, the most common cause of protozoal diarrhoea worldwide, in Mongolian gerbils. Plants were extracted in water, methanol and methanol:tetrahydrofuran. Gerbils were treated for ten days intragastrically twice a day, with the dose of 40 mg of the extract per 100 g of body weight. Drug metronidazole was used as a negative control. Gerbils' faeces were taken every day and examined by flotation method, the number of shed cysts were counted using a haemocytometer. After gerbils' sacrifice and dissection, their duodena were then processed for examination using histological sectioning and scanning electron microscopy. The antigiardial activity was evaluated by the course of cyst shedding throughout the entire experiment. A significant decline in cyst shedding, evaluated by linear regression was found in gerbils treated with the aqueous extract. Our results indicate that the aqueous extract of P. betle shows giardicidal effects.
Citace poskytuje Crossref.org
Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model
The course of experimental giardiasis in Mongolian gerbil
