PBP2a in β-Lactam-Resistant Laboratory Mutants and Clinical Isolates: Disruption Versus Reduced Penicillin Affinity
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
29195053
DOI
10.1089/mdr.2017.0302
Knihovny.cz E-resources
- Keywords
- PBP2a, Streptococcus pneumoniae, mosaic gene, penicillin resistance,
- MeSH
- Genes, Bacterial genetics MeSH
- Bacterial Proteins genetics MeSH
- beta-Lactams pharmacology MeSH
- DNA, Bacterial genetics MeSH
- Humans MeSH
- Microbial Sensitivity Tests methods MeSH
- Mutation genetics MeSH
- Penicillins pharmacology MeSH
- Peptide Synthases genetics MeSH
- Penicillin-Binding Proteins genetics MeSH
- Penicillin Resistance genetics MeSH
- Streptococcus pneumoniae drug effects genetics MeSH
- Carrier Proteins genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- South Africa MeSH
- Hungary MeSH
- Names of Substances
- Bacterial Proteins MeSH
- beta-Lactams MeSH
- DNA, Bacterial MeSH
- Penicillins MeSH
- penicillin-binding protein 2a, Streptococcus MeSH Browser
- Peptide Synthases MeSH
- Penicillin-Binding Proteins MeSH
- Carrier Proteins MeSH
Alterations in PBP2a have been recognized in cefotaxime-resistant laboratory mutants and β-lactam-resistant clinical isolates of Streptococcus pneumoniae. DNA sequencing revealed fundamental differences between these two settings. Internal stop codons in pbp2a occurred in all three laboratory mutants analyzed, caused by a mutation in pbp2a of mutant C604, and tandem duplications within pbp2a resulting in premature stop codons in another two mutants C403 and C406. In contrast, mosaic PBP2a genes were observed in several penicillin-resistant clinical isolates from South Africa, the Czech Republic, Hungary, and in the clone Poland23F-16, with sequence blocks diverging from sensitive strains by over 4%. Most of these pbp2a variants except pbp2a from the South African strain contained sequences related to pbp2a of Streptococcus mitis B6, confirming that this species serves as reservoir for penicillin-resistance determinants.
BA Impfstoffe GmbH Ibbenbüren Germany
Department of Medical Microbiology German National Reference Center for Streptococci Aachen Germany
Department of Microbiology University of Kaiserslautern Kaiserslautern Germany
School of Engineering University of Applied Sciences Saarbrücken Germany
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