This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer's molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl; required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production.

Biology Centre The Czech Academy of Sciences 5 v i Branisovska 31 370 05 Ceske Budejovice Czech Republic; University of South Bohemia Faculty of Science Branisovska 31 370 05 Ceske Budejovice Czech Republic

Faculty of Chemistry Brno University of Technology Purkynova 118 612 00 Brno Czech Republic

Institute of Chemistry NAWI Graz University of Graz Heinrichstrasse 28 3 8010 Graz Austria; ARENA Arbeitsgemeinschaft für Ressourcenschonende and Nachhaltige Technologien Inffeldgasse 21b 8010 Graz Austria

Institute of Scientific Instruments Academy of Sciences of the Czech Republic Vvi Kralovopolska 147 612 64 Brno Czech Republic

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