The rate of chromosome segregation errors that emerge during meiosis I in the mammalian female germ line are known to increase with maternal age; however, little is known about the underlying molecular mechanism. The objective of this study was to analyze meiotic progression of mouse oocytes in relation to maternal age. Using the mouse as a model system, we analyzed the timing of nuclear envelope breakdown and the morphology of the nuclear lamina of oocytes obtained from young (2 months old) and aged females (12 months old). Oocytes obtained from older females display a significantly faster progression through meiosis I compared to the ones obtained from younger females. Furthermore, in oocytes from aged females, lamin A/C structures exhibit rapid phosphorylation and dissociation. Additionally, we also found an increased abundance of MPF components and increased translation of factors controlling translational activity in the oocytes of aged females. In conclusion, the elevated MPF activity observed in aged female oocytes affects precocious meiotic processes that can multifactorially contribute to chromosomal errors in meiosis I.

Department of Cell Biology Faculty of Science Charles University Prague Albertov 6 128 43 Prague Czech Republic

Department of Genetics and Microbiology Faculty of Science Charles University Prague Albertov 6 128 43 Prague Czech Republic

Laboratory of Biochemistry and Molecular Biology of Germ Cells Institute of Animal Physiology and Genetics CAS Rumburska 89 277 21 Libechov Czech Republic

Laboratory of early mammalian development Department of Molecular Biology and Genetics Faculty of Science University of South Bohemia Branisovska 1760 370 05 Ceske Budejovice Czech Republic

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