Development and testing of a new tick-borne encephalitis virus vaccine candidate for veterinary use
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30337175
DOI
10.1016/j.vaccine.2018.10.034
PII: S0264-410X(18)31400-2
Knihovny.cz E-resources
- Keywords
- Alimentary infection, Sheep, Tick-borne encephalitis, Vaccine, Veterinary vaccine,
- MeSH
- Cell Culture Techniques MeSH
- Formaldehyde pharmacology MeSH
- Immunogenicity, Vaccine MeSH
- Vaccines, Inactivated administration & dosage immunology MeSH
- Encephalitis, Tick-Borne prevention & control veterinary MeSH
- Lactation MeSH
- Milk virology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Antibodies, Neutralizing blood immunology MeSH
- Sheep MeSH
- Polymerase Chain Reaction MeSH
- Antibodies, Viral blood immunology MeSH
- RNA, Viral analysis blood MeSH
- Viral Vaccines administration & dosage immunology MeSH
- Encephalitis Viruses, Tick-Borne MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Formaldehyde MeSH
- Vaccines, Inactivated MeSH
- Antibodies, Neutralizing MeSH
- Antibodies, Viral MeSH
- RNA, Viral MeSH
- Viral Vaccines MeSH
In tick-borne encephalitis (TBE) endemic areas, consumption of unpasteurized milk or milk products from grazing domestic ruminants (goats, cattle, and sheep) represents a risk of TBE virus (TBEV) infection for humans. In addition to vaccination of humans, human alimentary TBEV infections can be avoided by pasteurizing milk or by vaccination of the ruminants. However, there is presently no TBEV vaccine for veterinary use. Here, we developed a new veterinary TBE vaccine candidate based on cell culture-derived, purified, and formaldehyde-inactivated TBEV (strain Hypr). The safety and immunogenicity of the vaccine was evaluated in mice and sheep and was well-tolerated while eliciting the production of high levels of virus-neutralizing antibodies. Vaccination provided full protection against lethal TBE in mice, prevented development of viremia in sheep and presence of TBEV in milk of lactating ewes. This vaccine is a good candidate for immunization of ruminants to prevent alimentary milk-borne TBEV infections in humans.
Bioveta Inc Ivanovice na Hane Czech Republic
Department of Virology Veterinary Research Institute Brno Czech Republic
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