Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30452988
DOI
10.1016/j.ijbiomac.2018.11.161
PII: S0141-8130(18)35444-8
Knihovny.cz E-zdroje
- Klíčová slova
- CD81, CD9, Cattle, Embryos, Fertilization, Oocytes, Pig, Tetraspanins,
- MeSH
- antigeny CD81 metabolismus MeSH
- antigeny CD9 metabolismus MeSH
- buněčné linie MeSH
- embryo savčí cytologie metabolismus MeSH
- fertilizace in vitro účinky léků MeSH
- metafáze účinky léků MeSH
- myši inbrední BALB C MeSH
- oocyty cytologie metabolismus MeSH
- partenogeneze účinky léků MeSH
- prasata MeSH
- protilátky farmakologie MeSH
- skot MeSH
- stadium rýhování vajíčka cytologie účinky léků MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny CD81 MeSH
- antigeny CD9 MeSH
- protilátky MeSH
Tetraspanins are multifunctional molecules located in specific microdomains on the plasma membrane. Thanks to their ability to form networks with other proteins they can participate in many cellular functions. Tetraspanins are part of the interactive network in gametes; however, their precise role in fertilization is not yet clear. The aim of this study was to compare the localization of CD9 and CD81 tetraspanins during oocyte maturation and early development of the embryos in bovine and porcine model. CD9 was detected on the oocyte plasma membrane and vesicles in the perivitelline space of bovine oocytes and embryos. We suggest that CD9 could be a component involved in transzonal projections. Based on the results of in vitro fertilization assay, CD9 and CD81 seem to be part of a more complex fusion network on the plasma membrane of bovine oocytes. On the other hand, both tetraspanins showed a clustered expression pattern on the plasma membrane and inner margin of zona pellucida (ZP) in porcine oocytes and embryos. We found a new species-specific pattern of CD9 and CD81 distribution in ZP which could reflect their specialized role in processes associated with cell adhesion and intercellular communication upon fertilization.
Laboratory of Reproductive Biology Institute of Biotechnology CAS v v i BIOCEV Vestec Czech Republic
Citace poskytuje Crossref.org
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