Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
30452988
DOI
10.1016/j.ijbiomac.2018.11.161
PII: S0141-8130(18)35444-8
Knihovny.cz E-resources
- Keywords
- CD81, CD9, Cattle, Embryos, Fertilization, Oocytes, Pig, Tetraspanins,
- MeSH
- Tetraspanin 28 metabolism MeSH
- Tetraspanin 29 metabolism MeSH
- Cell Line MeSH
- Embryo, Mammalian cytology metabolism MeSH
- Fertilization in Vitro drug effects MeSH
- Metaphase drug effects MeSH
- Mice, Inbred BALB C MeSH
- Oocytes cytology metabolism MeSH
- Parthenogenesis drug effects MeSH
- Swine MeSH
- Antibodies pharmacology MeSH
- Cattle MeSH
- Cleavage Stage, Ovum cytology drug effects MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Tetraspanin 28 MeSH
- Tetraspanin 29 MeSH
- Antibodies MeSH
Tetraspanins are multifunctional molecules located in specific microdomains on the plasma membrane. Thanks to their ability to form networks with other proteins they can participate in many cellular functions. Tetraspanins are part of the interactive network in gametes; however, their precise role in fertilization is not yet clear. The aim of this study was to compare the localization of CD9 and CD81 tetraspanins during oocyte maturation and early development of the embryos in bovine and porcine model. CD9 was detected on the oocyte plasma membrane and vesicles in the perivitelline space of bovine oocytes and embryos. We suggest that CD9 could be a component involved in transzonal projections. Based on the results of in vitro fertilization assay, CD9 and CD81 seem to be part of a more complex fusion network on the plasma membrane of bovine oocytes. On the other hand, both tetraspanins showed a clustered expression pattern on the plasma membrane and inner margin of zona pellucida (ZP) in porcine oocytes and embryos. We found a new species-specific pattern of CD9 and CD81 distribution in ZP which could reflect their specialized role in processes associated with cell adhesion and intercellular communication upon fertilization.
Laboratory of Reproductive Biology Institute of Biotechnology CAS v v i BIOCEV Vestec Czech Republic
References provided by Crossref.org
Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos
Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells
