The karyotype of Greek cobitid fish Cobitisstrumicae Karaman, 1955, from Lake Volvi, Greece, a representative of one of its two major intraspecific phylogenetic lineages, was analysed by means of sequential Giemsa-staining, C-banding, silver-staining, CMA3 fluorescence banding and also by in situ hybridization (FISH) with rDNA probe. The diploid chromosome number was 2n = 50, karyotype composed of 10 pairs of metacentric to submetacentric and 15 pairs of subtelocentric to acrocentric chromosomes. The nucleolus organizer regions (NORs) as revealed by Ag- and CMA3 staining and FISH were situated in the telomeric region of the fourth submetacentric chromosome pair. The chromosomes contained very low content of C-positive heterochromatin. No heteromorphic sex chromosomes were detected. This first karyotype report for any species of lineage Bicanestrinia Băcescu, 1962 shows a simple karyotype dominated by acrocentric chromosomes and possessing single NOR-bearing chromosome pair. Cytotaxonomic implications of this finding for the taxonomy of the genus Cobitis Linnaeus, 1758 are further discussed.

Department of Genetics Development and Molecular Biology Faculty of Sciences School of Biology Aristotle University of Thessaloniki University Campus 54124 Thessaloniki Greece Aristotle University of Thessaloniki Thessaloniki Greece

Department of Zoology and Fisheries Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences 165 00 Prague Kamýcká 129 Czech Republic Czech University of Life Sciences Prague Czech Republic

Institut de Biologie Paris Seine UMR 7138 Evolution Sorbonne Universités Case 5 7 quai St Bernard 75952 Paris cedex 05 Paris France Sorbonne Universités Paris France

Laboratory of Fish Genetics Institute of Animal Physiology and Genetics Academy of Sciences of Czech Republic 277 21 Liběchov Czech Republic Institute of Animal Physiology and Genetics Academy of Sciences of Czech Republic Libĕchov Czech Republic

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