Anti-inflammatory compound curcumin and mesenchymal stem cells in the treatment of spinal cord injury in rats
Jazyk angličtina Země Polsko Médium print
Typ dokumentu časopisecké články
PubMed
30624435
PII: 7838
Knihovny.cz E-zdroje
- MeSH
- antiflogistika farmakologie MeSH
- kurkumin farmakologie MeSH
- mezenchymální kmenové buňky cytologie MeSH
- modely nemocí na zvířatech MeSH
- obnova funkce účinky léků MeSH
- poranění míchy farmakoterapie MeSH
- potkani Wistar MeSH
- regenerace nervu účinky léků MeSH
- transplantace mezenchymálních kmenových buněk * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antiflogistika MeSH
- kurkumin MeSH
Spinal cord injury leads to a robust inflammatory response that is an unfavorable environment for stem cell implantation. In this study, we evaluated the effect of combined therapy of curcumin and mesenchymal stem cells (MSC) on behavioral recovery and tissue sparing, glial scar formation, axonal sprouting and inflammatory responses in a rat experimental model of spinal cord injury (SCI). Balloon-induced compression lesion was performed at thoracic (Th8-9) spinal level. Out of the four groups studied, two groups received curcumin on the surface of the spinal cord immediately after SCI and then once a week for 3 weeks together with an intraperitoneal daily curcumin injection for 28 days. The other two groups received saline. Seven days after SCI, human MSC were intrathecally implanted in one curcumin and one saline group. Both curcumin and curcumin combined with MSC treatment improved locomotor ability in comparison to the saline treated animals. The combined treatment group showed additional improvement in advanced locomotor performance. The combined therapy facilitated axonal sprouting, and modulated expression of pro-regenerative factors and inflammatory responses, when compared to saline and single treatments. These results demonstrate that preconditioning with curcumin, prior to the MSC implantation could have a synergic effect in the treatment of experimental SCI.
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
New York Medical College Westchester Medical Center Valhalla New York USA
New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats
