A redescription is provided of the adult, tritonymph and deutonymph life stages of the troglobitic Neobisium (Blothrus) slovacum Gulička, 1977, which is known from Slovakia and Hungary. Material examined included 35 previously deposited museum specimens and 15 newly collected specimens. In addition, the karyotype and distribution of 18S rDNA clusters are described, using fluorescence in situ hybridization (FISH). The male karyotype of N.slovacum comprises 69 chromosomes, with a predominance of biarmed chromosomes, and an X0 sex chromosome system. Two pairs of signals for 18S rDNA on biarmed chromosomes (submetacentric and metacentric) of different sizes were identified. The present study provides the first information about the distribution of these clusters in the arachnid order Pseudoscorpiones. The geographic distribution of the species is summarized and mapped. Neobisiumslovacum is endemic to the Slovak and Aggtelek Karst area in southern Slovakia and north-western Hungary, where it has been recorded from 16 caves. One of these, Hačavská cave (in Slovakia), is the northernmost locality known for any species of the subgenus Blothrus.

Department of Zoology Faculty of Natural Sciences Comenius University Mlynská dolina Ilkovičova 6 SK 842 15 Bratislava Slovakia Comenius University Bratislava Slovakia

Department of Zoology Faculty of Science Charles University Prague Viničná 7 CZ 128 44 Prague 2 Czech Republic Charles University Prague Czech Republic

Department of Zoology Institute of Biology and Ecology Faculty of Science P J Šafárik University Šrobárova 2 SK 041 54 Košice Slovakia Šafárik University Košice Slovakia

Slovak Caves Administration Železničná 31 SK 979 01 Rimavská Sobota Slovakia Slovak Caves Administration Rimavská Sobota Slovakia

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Beier M. (1936) Zwei neue Pseudoskorpione aus deutschen Kleinsäuger-Höhlen. Zoologischer Anzeiger 114(3–4): 85–87.

Beier M. (1963) Ordnung Pseudoscorpionidea (Afterskorpione). Bestimmungsbücher zur Bodenfauna Europas. Vol. 1. Akademie-Verlag, Berlin, 313 pp.

Chamberlin JC. (1931) The arachnid order Chelonethida. Stanford University Publications. Biological Sciences 7(1): 1–284.

Christophoryová J. (2009) Šťúriky (Pseudoscorpiones) čeľadí Chthoniidae a Neobisiidae jaskýň a ich vchodov na Slovensku. In: Littera P. (Ed.) Zborník recenzovaných príspevkov, Študentská vedecká konferencia, 22 April 2009, Bratislava.Vydavateľstvo Univerzity Komenského, Bratislava, 254–260.

Christophoryová J, Šťáhlavský F, Fedor P. (2011) An updated identification key to the pseudoscorpions (Arachnida: Pseudoscorpiones) of the Czech Republic and Slovakia. Zootaxa 2876: 35–48.

Ducháč V. (1996) Über Neobisium (Blothrus) slovacum (Pseudoscorpiones: Neobisidae [sic]) aus der Brzotín – Höhle in der Slowakischen Republik. Klapalekiana 32: 153–157.

Ducháč V. (1999) Merkmale zur Bestimmung der Art Neobisium (Blothrus) slovacum (Arachnida: Pseudoscorpiones). Entomologische Zeitschrift 109(4): 175–180.

Ducháč V. (2002) Eutroglobiontní štírci podrodu Blothrus (Pseudoscorpiones: Neobisium) Západních Karpát. PhD Thesis, Univerzita Palackého v Olomouci, Olomouc.

Ducháč V. (2004a) Male genitalia of eutroglobiotic pseudoscorpions Neobisiumslovacum, Neobisiumleruthi, and Neobisiumaueri. Mitteilungen des Internationalen Entomologischen Vereins 29(1–2): 51–57.

Ducháč V. (2004b) Immaturní stadia štírka Neobisium (Blothrus) slovacum (Pseudoscorpiones: Neobisiidae). Folia Faunistica Slovaca 9(5): 37–40. http://ffs.sk/09-2004/05-duchac/index.htm [Table and figures missing]

Ducháč V, Mlejnek R. (2000a) Neobisiumslovacum – nejsevernější eutroglobiontní štírek Evropy. Speleofórum 19: 48–49.

Ducháč V, Mlejnek R. (2000b) Records of the pseudoscorpion Neobisium (Blothrus) slovacum (Neobisiidae) in caves and chasms of the Slovak Karst. In: Mock A, Kováč Ľ, Fulín M. (Eds) Fauna jaskýň (Cave Fauna).East Slovakian Museum, Košice, 15–20.

Forman M, Nguyen P, Hula V, Král J. (2013) Sex chromosome pairing and extensive NOR polymorphism in Wadicosafidelis (Araneae: Lycosidae). Cytogenetic and Genome Research 141: 43–49. 10.1159/000351041 PubMed DOI

Gulička J. (1977a) Neobisium (Blothrus) slovacum sp. n., eine neue Art des blinden Höhlenafterskorpions aus der Slowakei (Pseudoscorpionida). Annotationes Zoologicae et Botanicae 117: 1–9.

Gulička J. (1977b) K otázke výskytu pravých troglobiontov v jaskyniach Slovenska. Slovenský Kras 15: 23–29.

Harrison SE, Guzik MT, Harvey MS, Austin AD. (2014) Molecular phylogenetic analysis of Western Australian troglobitic chthoniid pseudoscorpions (Pseudoscorpiones: Chthoniidae) points to multiple independent subterranean clades. Invertebrate Systematics 28(4): 386–400. 10.1071/IS14005 DOI

Harvey MS. (1991) Catalogue of the Pseudoscorpionida. Manchester University Press, Manchester, 726 pp.

Harvey MS. (2013) Pseudoscorpions of the World, version 3.0. Western Australian Museum. Available from: http://museum.wa.gov.au/catalogues-beta/pseudoscorpions

Judson MLI. (2007) A new and endangered species of the pseudoscorpion genus Lagynochthonius from a cave in Vietnam, with notes on chelal morphology and the composition of the Tyrannochthoniini (Arachnida, Chelonethi, Chthoniidae). Zootaxa 1627: 53–68.

Kárpáthegyi P. (2007) Pseudoscorpions of Hungary. Folia Historico Naturalia Musei Matraensis 31: 81–90. http://matramuzeum.hu/e107_files/public/docrep/081_090.pdf

Kotrbová J, Opatova V, Gardini G, Šťáhlavský F. (2016) Karyotype diversity of pseudoscorpions of the genus Chthonius (Pseudoscorpiones, Chthoniidae) in the Alps. Comparative Cytogenetics 10(3): 325–345. 10.3897/CompCytogen.v10i3.8906 PubMed DOI PMC

Kováč Ľ, Mock A, Ľuptáčik P, Nováková A, Papáč V, Višňovská Z, Hudec I, Stankovič J. (2010) Jaskynné mikroorganizmy a bezstavovce. In: Stankovič J, Cílek V, Schmelzová R. (Eds) Plešivecká planina.Jaskyne Plešiveckej planiny – svetové prírodné dedičstvo. Speleoklub Minotaurus. Slovenská speleologická spoločnosť, Liptovský Mikuláš, 143–149.

Lysenko V. (1972) Výzkum Plešivecké planiny ve Slovenském krasu v letech 1965–1968. Československý kras 21: 97–109.

Novák J. (2014) Notes on two species of the cavernicolous subgenus Neobisium (Blothrus) Schiödte, 1874 (Arachnida: Pseudoscorpiones) from Transylvania (Romania), with a key to the species of the Carpathian Mountains. Zootaxa 3796(2): 394–400. 10.11646/zootaxa.3796.2.11 PubMed DOI

Opatova V, Arnedo MA. (2014) Spiders on a hot volcanic roof: colonisation pathways and phylogeography of the Canary Islands endemic trap-door spider Titanidiopscanariensis (Araneae, Idiopidae). PLoS ONE 9(12): e115078. 10.1371/journal.pone.0115078 PubMed DOI PMC

Plíšková J, Kovařík F, Košulič O, Šťáhlavský F. (2016) Description of a new species of Heterometrus Ehrenberg, 1828 (Scorpiones: Scorpionidae) from Thailand with remarks about the utilization of cytogenetic data in taxonomy of the genus. Annales Zoologici 66(3): 467–476. 10.3161/00034541ANZ2016.66.3.011 DOI

Řezáč M, Arnedo MA, Opatova V, Musilová J, Řezáčová V, Král J. (2018) Taxonomic revision and insights into the speciation mode of the spider Dysderaerythrina species-complex (Araneae: Dysderidae): sibling species with sympatric distributions. Invertebrate Systematics 32(1): 10–54. 10.1071/IS16071 DOI

Sakamoto Y, Zacaro AA. (2009) LEVAN, an ImageJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes. Initial version. http://rsbweb.nih.gov/ij/

Sokolow I. (1926) Untersuchungen über die Spermatogenese bei den Arachniden. II: Über die Spermatogenese der Pseudoscorpione. Zeitschrift für Zellforschung und Mikroskopische Anatomie 3: 615–681. 10.1007/BF01094348 DOI

Šťáhlavský F, Christophoryová J, Henderickx H. (2013) A karyological study of four European species of Roncus (Pseudoscorpiones: Neobisiidae). European Journal of Entomology 110(3): 393–399. http://www.eje.cz/pdfs/110/3/393

Šťáhlavský F, Král J. (2004) Karyotype analysis and achiasmatic meiosis in pseudoscorpions of the family Chthoniidae (Arachnida: Pseudoscorpiones). Hereditas 140(1): 49–60. 10.1111/j.1601–5223.2004.01783.x PubMed DOI

Šťáhlavský F, Král J, Harvey MS, Haddad CR. (2006) A karyotype study on the pseudoscorpion families Geogarypidae, Garypinidae and Olpiidae (Arachnida: Pseudoscorpiones). European Journal of Entomology 103(2): 277–289. 10.14411/eje.2006.036 DOI

Šťáhlavský F, Král J, Harvey MS, Haddad CR. (2012a) The first cytogenetic characterization of atemnids: pseudoscorpions with the highest chromosome numbers (Arachnida: Pseudoscorpiones). Cytogenetic and Genome Research 137(1): 22–30. 10.1159/000339516 PubMed DOI

Šťáhlavský F, Opatova V, Just P, Lotz LN, Haddad CR. (2018a) Molecular technique reveals high variability of 18S rDNA distribution in harvestmen (Opiliones, Phalangiidae) from South Africa. Comparative Cytogenetics 12(1): 41–59. 10.3897/CompCytogen.v12i1.21744 PubMed DOI PMC

Šťáhlavský F, Štundlová J, Lowe G, Stockmann M, Kovařík F. (2018b) Application of cytogenetic markers in the taxonomy of flat rock scorpions (Scorpiones: Hormuridae), with the description of Hadogenesweygoldti sp. n. Zoologischer Anzeiger 273: 173–182. 10.1016/j.jcz.2018.01.007 DOI

Šťáhlavský F, Vítková M, Šlachta M, Janko K. (2012b) Karyotype evolution in pseudoscorpion family Neobisiidae (Arachnida: Pseudoscorpiones). Chromosome Research 20: 803–804.

Šťáhlavský F, Zeh JA, Zeh DW, Král J. (2009) Karyotypes of the Neotropical pseudoscorpions Semeiochernesarmiger and Cordylochernesscorpioides (Pseudoscorpiones: Chernetidae). Journal of Arachnology 37(3): 287–291. 10.1636/P08-86.1 DOI

Svojanovská H, Nguyen P, Hiřman M, Tuf IH, Wahab RA, Haddad CR, Šťáhlavský F. (2016) Karyotype evolution in harvestmen of the suborder Cyphophthalmi (Opiliones). Cytogenetic and Genome Research 148(2–3): 227–236. 10.1159/000445863 PubMed DOI

Troiano G. (1990) Karyotype and male meiosis of four species of Roncus L. Koch, 1873 (Pseudoscorpionida, Neobisiidae). Bollettino di Zoologia 57: 1–9. 10.1080/11250009009355666 DOI

Vlk L. (2001) Dolní Vrch. Slovenské múzeum ochrany prírody a jaskyniarstva, Liptovský Mikuláš, 137 pp.

Zaragoza JA, Šťáhlavský F. (2008) A new Roncus species (Pseudoscorpiones: Neobisiidae) from Montseny Natural Park (Catalonia, Spain), with remarks on karyology. Zootaxa 1693: 27–40. https://pdfs.semanticscholar.org/3863/df4b232b9fac01d44767e60ce4be089e9a49.pdf

Integrative Taxonomy Approach Reveals Cryptic Diversity within the Phoretic Pseudoscorpion Genus Lamprochernes (Pseudoscorpiones: Chernetidae)

Insights into the Karyotype Evolution of Charinidae, the Early-Diverging Clade of Whip Spiders (Arachnida: Amblypygi)