Multiple In vitro biological effects of phenolic compounds from Morus alba root bark
Language English Country Ireland Media print-electronic
Document type Journal Article
PubMed
31610262
DOI
10.1016/j.jep.2019.112296
PII: S0378-8741(19)33729-8
Knihovny.cz E-resources
- Keywords
- Anti-inflammatory, Antibacterial, Antiviral, Docking, Mulberry, Phenolic,
- MeSH
- Cell Line MeSH
- Diabetes Mellitus drug therapy metabolism MeSH
- Glucose metabolism MeSH
- Glycogen metabolism MeSH
- Hypoglycemic Agents pharmacology therapeutic use MeSH
- Insulin Resistance MeSH
- Liver drug effects metabolism MeSH
- Muscle, Skeletal drug effects metabolism MeSH
- Rats MeSH
- Plant Leaves MeSH
- Membrane Potential, Mitochondrial drug effects MeSH
- Morus * MeSH
- Mice MeSH
- AMP-Activated Protein Kinases metabolism MeSH
- Reactive Oxygen Species metabolism MeSH
- Plant Extracts pharmacology therapeutic use MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glucose MeSH
- Glycogen MeSH
- Hypoglycemic Agents MeSH
- AMP-Activated Protein Kinases MeSH
- Reactive Oxygen Species MeSH
- Plant Extracts MeSH
ETHNOPHARMACOLOGICAL RELEVANCE: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. AIM OF THE STUDY: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. MATERIALS AND METHODS: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. RESULTS: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 μg/mL, and EC50 values 0.93 and 1.61 μg/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 μg/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. CONCLUSIONS: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.
References provided by Crossref.org
Natural Phenolics Disrupt Microbial Communication by Inhibiting Quorum Sensing
Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
Prenylated Flavonoids in Topical Infections and Wound Healing
Berberine in Human Oncogenic Herpesvirus Infections and Their Linked Cancers
Natural Products-Derived Chemicals: Breaking Barriers to Novel Anti-HSV Drug Development
